Music-Box: Controlling microfluidic flow with customized melodies for point-of-care testing

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-06-02 DOI:10.1016/j.bios.2025.117653

Bangfeng Wang , Yangcheng Liu , Shunji Li , Ying Zhang , Dongjuan Chen , Mingxuan Li , Zetai Liu , Peng Chen , Yiwei Li , Xiaojun Feng , Bi-Feng Liu , Wei Lin

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引用次数: 0

Abstract

The global prevalence of Human Papillomavirus (HPV) underscores an urgent need for solutions of accessible and rapid diagnostics. Although microfluidic chips have paved a promising pathway for achieving point-of-care-testing (POCT), the reliance on bulky and high-cost pumps has restricted their applicability in decentralized settings. Here, we present "Music-Box", a system leveraging a novel actuation mechanism based on the asymmetric motion of the speaker to achieve precise microfluidic control. By eliminating the need for fixed unidirectional structures, this system enables bidirectional fluid manipulation with high precision. Coupled with a smartphone, Music-Box offers a sample-to-result workflow for HPV nucleic acid detection. Clinical verification using 50 hospital-sourced samples reveals that the device has a 97.3 % sensitivity and 100 % specificity for HPV detection. By integrating portability, affordability, and precision, Music-Box redefines the method of POCT, enabling scalable diagnostics not only under resource-limited settings but also adaptable to scenarios of various infectious diseases.

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音乐盒：控制微流体流动与定制的旋律点护理测试

人类乳头瘤病毒（HPV）的全球流行突出表明迫切需要可获得和快速诊断的解决方案。尽管微流控芯片为实现护理点检测（POCT）铺平了一条充满希望的道路，但对笨重和高成本泵的依赖限制了它们在分散环境中的适用性。在这里，我们提出了“音乐盒”，一个利用基于扬声器不对称运动的新型驱动机制来实现精确微流体控制的系统。通过消除对固定单向结构的需求，该系统可以实现高精度的双向流体操纵。再加上智能手机，音乐盒为HPV核酸检测提供了一个从样本到结果的工作流程。临床验证使用50医院来源的样本显示，该设备有97.3%的灵敏度和100%的特异性HPV检测。通过整合可移植性、可负担性和精确性，Music-Box重新定义了POCT方法，不仅在资源有限的环境下实现了可扩展的诊断，而且适用于各种传染病的情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.