Serological insights into MERS-CoV dynamics of antibody responses during acute and convalescent phases and their clinical relevance for diagnostics and immunity

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-06-02 DOI:10.1016/j.jiph.2025.102854

Khalid J. Shrwani , Ahmad M. Alharbi , Waleed H. Mahallawi , Ayed Y. Asiri , Abdullah M. Assiri , Abdullah Algaissi , Anwar M. Hashem , Radi Alsafi , Mohammed A. Jubran , Eyad Gadour , Soha A. Alghamdi , Ezzuddin A. Okmi , Nabil Dhayhi , Hassan A. Asiri , Zaki M. Eisa , Nigel Cunliffe , Qibo Zhang

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引用次数: 0

Abstract

Introduction

Middle East respiratory syndrome (MERS) is a zoonotic viral respiratory disease caused by the Middle East respiratory syndrome coronavirus (MERS-CoV), associated with severe clinical outcomes and high mortality.

Objectives

Our study examined the kinetics of anti-MERS-CoV IgM and IgG antibodies during the acute and convalescent phases of infection, focusing on their correlations with clinical variables such as age and viral load.

Methods

Serum samples were collected from PCR-confirmed MERS-CoV patients (n = 23) during both phases and compared to healthy controls (n = 23) using validated ELISA-based assays.

Results

IgM levels peaked in the acute phase and declined significantly in the convalescent phase, while IgG levels were sustained and markedly higher during recovery. Correlation analyses revealed positive relationships between antibody levels and patient age (acute IgM: r = 0.56, p < 0.01; convalescent IgG: r = 0.59, p < 0.01) and viral loads (acute IgM: r = 0.97, p < 0.0001; acute IgG: r = 0.87, p < 0.0001; convalescent IgG: r = 0.91, p < 0.0001). These results concluded that age-associated enhancements in antibody responses and the role of humoral immunity in viral clearance.

Conclusion

The current study highlights the diagnostic value of anti-MERS-CoV IgM and IgG measurements and their utility in developing therapeutic and vaccine strategies adapted to high-risk populations.

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急性期和恢复期MERS-CoV抗体反应的血清学动态及其诊断和免疫的临床相关性

中东呼吸综合征（MERS）是一种由中东呼吸综合征冠状病毒（MERS- cov）引起的人畜共患病毒性呼吸道疾病，具有严重的临床结局和高死亡率。目的研究mers - cov抗IgM和IgG抗体在感染急性期和恢复期的动力学，重点研究其与年龄和病毒载量等临床变量的相关性。方法采集pcr确诊的MERS-CoV患者（n = 23）两期血清样本，并与健康对照（n = 23）采用经验证的elisa法进行比较。结果igm水平在急性期达到高峰，恢复期显著下降，而IgG水平在恢复期持续升高。相关分析显示抗体水平与患者年龄呈正相关(急性IgM: r = 0.56,p <； 0.01；恢复期IgG: r = 0.59,p <； 0.01)和病毒载量(急性期IgM: r = 0.97,p <； 0.0001；急性IgG: r = 0.87,p <； 0.0001；恢复期IgG: r = 0.91,p <； 0.0001)。这些结果表明，年龄相关的抗体反应增强和体液免疫在病毒清除中的作用。结论本研究强调了抗mers - cov IgM和IgG检测的诊断价值及其在制定适合高危人群的治疗和疫苗策略中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.