Mapping the zebrafish regenerative heart

IF 5.9 3区农林科学 Q1 VETERINARY SCIENCES

Lab Animal Pub Date : 2025-06-03 DOI:10.1038/s41684-025-01569-6

Alexandra Le Bras

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引用次数: 0

Abstract

While adult mammals have an extremely limited capacity to repair lost or damaged heart tissue, many adult fish, including zebrafish, can successfully regenerate their hearts after injury. Uncovering the mechanisms of zebrafish heart regeneration could open new avenues to regenerate human hearts and prevent heart failure. In a new study, researchers combined the use of spatial transcriptomics (Stereo-seq) and single-cell RNA-sequencing (scRNA-seq) to generate a spatially-resolved molecular and cellular atlas of the regenerating zebrafish heart across eight stages, including pre-injury, 6 hours post-amputation (hpa) of the ventricular apex, 12 hpa, 1 day post-amputation (dpa), 3 dpa, 7 dpa, 14 dpa, and 28 dpa. Using this comprehensive atlas, they characterized the dynamic changes in cell type composition throughout cardiac regeneration, including proliferating macrophages, pro-regenerative fibroblasts and regenerating cardiomyocytes. The study also identified tpm4a as a critical gene for cardiomyocyte re-differentiation during the regeneration process. Finally, the researchers created a ‘virtual regenerating heart’, encompassing both spatial information and time, which can be accessed here: https://db.cngb.org/stomics/zebrafish_VRH/

Original reference: Li, L. et al. Nat. Commun. 16, 3716 (2025)

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绘制斑马鱼再生心脏图

虽然成年哺乳动物修复丢失或受损心脏组织的能力极其有限，但许多成年鱼类，包括斑马鱼，在受伤后可以成功地再生心脏。揭示斑马鱼心脏再生的机制可以为人类心脏再生和预防心力衰竭开辟新的途径。在一项新的研究中，研究人员将空间转录组学（Stereo-seq）和单细胞rna测序（scRNA-seq）结合使用，生成了再生斑马鱼心脏的8个阶段的空间分辨分子和细胞图谱，包括损伤前、截肢后6小时（hpa）、12小时、截肢后1天（dpa）、3小时、7小时、14小时和28小时。利用这一综合图谱，他们表征了心脏再生过程中细胞类型组成的动态变化，包括增殖性巨噬细胞、促再生成纤维细胞和再生心肌细胞。该研究还发现tpm4a是再生过程中心肌细胞再分化的关键基因。最后，研究人员创造了一个“虚拟再生心脏”，包括空间信息和时间，可以在这里访问：https://db.cngb.org/stomics/zebrafish_VRH/Original参考文献：Li， L.等人。公共法典，163716 （2025）

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lab Animal 农林科学-兽医学

CiteScore

0.60

自引率

2.90%

发文量

181

审稿时长

>36 weeks

期刊介绍： LabAnimal is a Nature Research journal dedicated to in vivo science and technology that improves our basic understanding and use of model organisms of human health and disease. In addition to basic research, methods and technologies, LabAnimal also covers important news, business and regulatory matters that impact the development and application of model organisms for preclinical research. LabAnimal's focus is on innovative in vivo methods, research and technology covering a wide range of model organisms. Our broad scope ensures that the work we publish reaches the widest possible audience. LabAnimal provides a rigorous and fair peer review of manuscripts, high standards for copyediting and production, and efficient publication.