Topoisomerase inhibitory activity of ethanolic extracts from botanicals Coptis chinensis and Salvia officinalis.

Abstract

Introduction. DNA topoisomerases are essential enzymes that allow cells to effectively manage the topological states of DNA. Due to the ubiquitous nature of their functions, topoisomerases have become promising treatment targets for various conditions, ranging from microbial infections to cancer.Hypothesis. The botanicals, Coptis chinensis (Chinese goldthread) and Salvia officinalis (common sage), are herbs that boast a long history of traditional use for their effectiveness in treating a myriad of health concerns, including microbial infections and cancer, which could be associated with topoisomerase inhibitory activity.Aim. This study sought to evaluate the antimicrobial and anticancer properties of these botanical extracts and determine if this activity was due to the presence of anti-topoisomerase activity.Methodology. Using various bacterial genera, vaccinia virus, cancerous cell lines and topoisomerase activity assays, the activity of these extracts was evaluated.Results. This study demonstrated that ethanolic extracts of these botanicals had potent anti-Gram-positive bacterial activity, antiviral activity and anticancer activity. Furthermore, this activity likely correlated with the ability of the extracts to inhibit topoisomerases II and IV and for Salvia officinalis, topoisomerase I.Conclusion. These results support the potential therapeutic value of C. chinensis and S. officinalis for the treatment of health concerns.