The Role of Histopathological Examination in the Diagnosis of Cutaneous Sporotrichosis Caused by Sporothrix globosa: A Retrospective Analysis and Reassessment.

IF 3.1 2区医学 Q1 DERMATOLOGY

Mycoses Pub Date : 2025-06-01 DOI:10.1111/myc.70072

Liuyu Li, Jiajia Li, Zehu Liu, Xiujiao Xia

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引用次数: 0

Abstract

Background: Due to the paucity of fungal elements in the tissue, the sensitivity of histopathological tests for the diagnosis of cutaneous sporotrichosis remains low, particularly for low-virulent Sporothrix species.

Objectives: We retrospectively analysed and re-evaluated the role of histopathological examination in the diagnosis of cutaneous sporotrichosis caused by Sporothrix globosa.

Methods: Retrospective analysis and pathological reassessment were conducted for 69 sporotrichosis cases caused by S. globosa between 2013 and 2024. Statistical analysis using prevalence ratios was conducted to characterise the clinicopathological and epidemiological aspects.

Results: Histopathological examination revealed mixed inflammatory cell infiltration and granulomatous changes in all cases. Periodic acid-Schiff (PAS) staining was performed in 29 tissue specimens, with positivity observed in 4 cases (13.8%, 4/29). Following supplemental staining and re-evaluation of the slides, PAS staining revealed positivity in 50.7% (35/69) of cases, demonstrating yeast forms (91.4%, 32/35) with asteroid bodies (n = 2) and rare hyphae (n = 1).

Conclusions: Currently, the role of histopathological examination in the diagnosis of sporotrichosis is limited. Our study showed that the frequency of detecting S. globosa based on histopathological examination is considerably high. Nevertheless, achieving high positive rates necessitates the collaborative efforts of experienced mycologists. The predominance of suppurative granulomas or neutrophils is related to the presence of the fungus in tissue sections from human patients.

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组织病理学检查在全球孢子丝菌引起的皮肤孢子菌病诊断中的作用：回顾性分析和再评价。

背景：由于组织中真菌成分的缺乏，组织病理学检查诊断皮肤孢子菌病的敏感性仍然很低，特别是对低毒力的孢子菌种。目的：回顾性分析并重新评价组织病理学检查在全球孢子丝菌引起的皮肤孢子菌病诊断中的作用。方法：回顾性分析2013 - 2024年69例球形葡萄球菌孢子虫病病例并进行病理再评估。使用患病率进行统计分析，以确定临床病理和流行病学方面的特征。结果：组织病理检查均可见炎性细胞浸润及肉芽肿样改变。29例组织标本行PAS周期性染色，阳性4例（13.8%,4/29）。在补充染色和重新评估玻片后，PAS染色显示50.7%（35/69）的病例呈阳性，显示酵母形式（91.4%,32/35），小行星体（n = 2）和罕见菌丝（n = 1）。结论：目前，组织病理学检查在孢子虫病诊断中的作用有限。我们的研究表明，基于组织病理学检查的全球葡萄球菌检测频率相当高。然而，实现高阳性率需要经验丰富的真菌学家的合作努力。化脓性肉芽肿或中性粒细胞的优势与人类患者组织切片中真菌的存在有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycoses 医学-皮肤病学

CiteScore

10.00

自引率

8.20%

发文量

143

审稿时长

6-12 weeks

期刊介绍： The journal Mycoses provides an international forum for original papers in English on the pathogenesis, diagnosis, therapy, prophylaxis, and epidemiology of fungal infectious diseases in humans as well as on the biology of pathogenic fungi. Medical mycology as part of medical microbiology is advancing rapidly. Effective therapeutic strategies are already available in chemotherapy and are being further developed. Their application requires reliable laboratory diagnostic techniques, which, in turn, result from mycological basic research. Opportunistic mycoses vary greatly in their clinical and pathological symptoms, because the underlying disease of a patient at risk decisively determines their symptomatology and progress. The journal Mycoses is therefore of interest to scientists in fundamental mycological research, mycological laboratory diagnosticians and clinicians interested in fungal infections.