Profile of Salmonella Isolates in Bullfrog Production: PFGE, MLST, and Antimicrobial Resistance

IF 2.1 4区农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of food protection Pub Date : 2025-05-31 DOI:10.1016/j.jfp.2025.100550

Evelyn Cristine Silva , Sthéfany da Cunha Dias , Priscila Cristina Costa , Monique Ribeiro Tiba Casas , Ricardo Seiti Yamatogi , João Pessoa Araújo Junior , Marcus Vinícius Coutinho Cossi , Fábio Sossai Possebon

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引用次数: 0

Abstract

The continued development of bullfrog farming holds great potential for expansion. However, these animals serve as reservoirs for various pathogens, including Salmonella, posing a risk to food safety and public health. Therefore, the primary objective of this study was to identify potential sources of Salmonella contamination in a bullfrog production facility in Minas Gerais, Brazil, by characterizing isolates through Pulsed-Field Gel Electrophoresis (PFGE), Multilocus Sequence Typing (MLST), and antimicrobial resistance profiling. A total of eight Salmonella isolates were analyzed, comprising six isolates from bullfrog carcasses and two from breeding tanks. PFGE characterization revealed four distinct profiles, with a clonal relationship among isolates belonging to the same serovar, except for S. Newport, where the profile of the breeding tank isolate differed from that of the carcass isolates. MLST analysis identified three sequence types (STs): ST 32, ST 614, and ST 6855, corresponding to S. Infantis, S. Newport, and S. 6,8:i:-, respectively. Regarding antimicrobial resistance, one isolate was resistant to azithromycin, two were resistant to neomycin, and three were resistant to ciprofloxacin. All isolates were susceptible to cephalexin, meropenem, cefotaxime, ceftriaxone, ampicillin, tobramycin, and cephalothin. The presence of distinct PFGE profiles among isolates of the same serovar suggests multiple sources of pathogen contamination within the production chain, raising significant sanitary concerns. The identified sequence types (STs) are of public health relevance, highlighting the pathogenic potential of these isolates. While most isolates were susceptible to the tested antimicrobials, the detection of azithromycin resistance is particularly concerning. The combined PFGE and MLST data indicate potential cross-contamination within the production chain, emphasizing the need for stringent control measures.

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牛蛙生产中沙门氏菌分离株概况：PFGE、MLST和抗菌素耐药性。

牛蛙养殖的持续发展具有巨大的发展潜力。然而，这些动物是包括沙门氏菌在内的各种病原体的宿主，对食品安全和公众健康构成威胁。因此，本研究的主要目的是通过脉冲场凝胶电泳（PFGE）、多位点序列分型（MLST）和抗菌素耐药性分析来鉴定巴西米纳斯吉拉斯州牛蛙生产设施中沙门氏菌污染的潜在来源。共分离出8株沙门氏菌，其中6株分离自牛蛙尸体，2株分离自养殖池。PFGE鉴定显示了四种不同的特征，在属于同一血清型的分离株之间具有克隆关系，除了S. Newport，其繁殖池分离株的特征与胴体分离株的特征不同。MLST分析鉴定出3种序列类型（ST）： ST 32、ST 614和ST 6855，分别对应S. Infantis、S. Newport和S. 6,8:i:-。耐药性方面，1株对阿奇霉素耐药，2株对新霉素耐药，3株对环丙沙星耐药。所有分离株均对头孢氨苄、美罗培南、头孢噻肟、头孢曲松、氨苄西林、妥布霉素和头孢肽敏感。在同一血清型的分离株中存在不同的PFGE谱，这表明在生产链中存在多种病原体污染来源，引起了重大的卫生问题。鉴定的序列类型（STs）具有公共卫生相关性，突出了这些分离株的致病潜力。虽然大多数分离株对所测试的抗菌素敏感，但阿奇霉素耐药性的检测尤其令人担忧。综合PFGE和MLST数据表明，在生产链中存在潜在的交叉污染，强调需要采取严格的控制措施。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of food protection 工程技术-生物工程与应用微生物

CiteScore

4.20

自引率

5.00%

发文量

296

审稿时长

2.5 months

期刊介绍： The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with: Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain; Microbiological food quality and traditional/novel methods to assay microbiological food quality; Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation; Food fermentations and food-related probiotics; Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers; Risk assessments for food-related hazards; Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods; Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.