Comprehensive analysis of mRNA and miRNA expression profiles in follicular development in quail.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

British Poultry Science Pub Date : 2025-08-01 Epub Date: 2025-06-02 DOI:10.1080/00071668.2025.2451263

X Zhang, L Liu, Y Liu, Y Liu, J Yang

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引用次数: 0

Abstract

1. Ovarian follicle development is a key determinant of egg production in quail which is regulated by a dynamic and stage-specific genetic program. However, most follicle-related genes and miRNAs have not yet been identified in quail.2. This study applied transcriptome sequencing to dissect mRNA and miRNA expression patterns in quail ovarian follicles at distinct developmental stages: the pre-selection (SYF), the post-selection (F5) and the pre-ovulatory follicles (F1) in egg-laying birds. Comparative analyses identified 362 differentially-expressed mRNAs and 13 miRNAs in the F1 vs. F5 comparison, 549 and 72 in the F5 vs. SYF comparison and 1833 and 55 in the F1 vs. SYF comparison, respectively.3. Significant differentially-expressed mRNAs implicated in follicle development included VCAN, FGFR3, ALDH1A1, NR0B1,ZP2 and SMOC1. Other significant miRNAs were represented by ga-miR-460b-5p, gga-miR-1552-5p, gga-miR-125b-5p, gga-miR-135a-5p, gga-miR-122-5p, gga-miR-29b-3p and ggga-miR-138-1-3p.4. Enrichment analyses using GO and KEGG databases highlighted the influence of the extracellular matrix (ECM), cell adhesion molecules (CAMs), TGF-β and calcium signalling pathways and the steroid synthesis pathway on follicular development.5. Additionally, the miRNA-mRNA interaction network pinpointed the regulatory roles of specific differentially expressed miRNAs (DE miRNAs), including gga-miR-1306-5p, gga-miR-133a-3p, gga-miR-133c-3p, gga-miR-214, gga-miR-214b-5p and gga-miR-34a-5p.

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鹌鹑卵泡发育中mRNA和miRNA表达谱的综合分析。

1. 卵巢卵泡发育是鹌鹑产蛋的关键决定因素，受动态和阶段特异性遗传程序的调节。然而，大多数卵泡相关基因和mirna尚未在鹌鹑中被鉴定出来。本研究应用转录组测序技术，分析了鹌鹑卵泡在不同发育阶段的mRNA和miRNA表达模式：蛋禽的预选择（SYF）、后选择（F5）和排卵前卵泡（F1）。比较分析在F1与F5的比较中鉴定出362个差异表达mrna和13个mirna，在F5与SYF的比较中鉴定出549个和72个，在F1与SYF的比较中鉴定出1833个和55个。与卵泡发育相关的显著差异表达mrna包括VCAN、FGFR3、ALDH1A1、NR0B1、ZP2和SMOC1。其他重要的mirna包括ga-miR-460b-5p、gga-miR-1552-5p、gga-miR-125b-5p、gga-miR-135a-5p、gga-miR-122-5p、gga-miR-29b-3p和ggga- mir -138-1-3p。利用GO和KEGG数据库进行富集分析，突出了细胞外基质（ECM）、细胞粘附分子（CAMs）、TGF-β和钙信号通路以及类固醇合成途径对卵泡发育的影响。此外，miRNA-mRNA相互作用网络确定了特定差异表达miRNAs （DE miRNAs）的调节作用，包括gga-miR-1306-5p， gga-miR-133a-3p, gga-miR-133a-3p, gga-miR-214， gga-miR-214b-5p和gga-miR-34a-5p。

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .