Mengli Xie, Baoping Zhao, Jianxiang Teng, Long Yang, Zhen Wang, Shun'en Xu, Zhanyu Wu, Chuan Ye
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引用次数: 0
Abstract
Background: Platelet-rich plasma (PRP) is extensively used in regenerative medicine; however, its preparation lacks standardization. The difference in rotor angle between fixed-angle and horizontal rotors influences PRP quality. This study aims to compare the effects of these two rotor types on PRP preparation.
Methods: A total of 15 subjects were enrolled in the study, with each subject donating 80 ml of whole blood, which was then divided into two separate tubes, each containing 40 ml. PRP was prepared using two rounds of centrifugation with both fixed-angle and horizontal rotors under optimal parameters. Platelet recovery, concentration, growth factor content, and effects on cell co-culture were assessed.
Results: The fixed-angle centrifuge demonstrated higher platelet recovery efficiency, increased platelet content, and greater PDGF-BB levels in the PRP, with no significant change in CD62P expression. Co-culture assays showed enhanced cell proliferation and migration with PRP prepared using the fixed-angle rotor compared to the horizontal rotor.
Conclusions: Under appropriate centrifugation parameters, the fixed-angle centrifuge can extract higher quality PRP in a shorter time.
Level of evidence ii: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
期刊介绍：
Aesthetic Plastic Surgery is a publication of the International Society of Aesthetic Plastic Surgery and the official journal of the European Association of Societies of Aesthetic Plastic Surgery (EASAPS), Società Italiana di Chirurgia Plastica Ricostruttiva ed Estetica (SICPRE), Vereinigung der Deutschen Aesthetisch Plastischen Chirurgen (VDAPC), the Romanian Aesthetic Surgery Society (RASS), Asociación Española de Cirugía Estética Plástica (AECEP), La Sociedad Argentina de Cirugía Plástica, Estética y Reparadora (SACPER), the Rhinoplasty Society of Europe (RSE), the Iranian Society of Plastic and Aesthetic Surgeons (ISPAS), the Singapore Association of Plastic Surgeons (SAPS), the Australasian Society of Aesthetic Plastic Surgeons (ASAPS), the Egyptian Society of Plastic and Reconstructive Surgeons (ESPRS), and the Sociedad Chilena de Cirugía Plástica, Reconstructiva y Estética (SCCP).
Aesthetic Plastic Surgery provides a forum for original articles advancing the art of aesthetic plastic surgery. Many describe surgical craftsmanship; others deal with complications in surgical procedures and methods by which to treat or avoid them. Coverage includes "second thoughts" on established techniques, which might be abandoned, modified, or improved. Also included are case histories; improvements in surgical instruments, pharmaceuticals, and operating room equipment; and discussions of problems such as the role of psychosocial factors in the doctor-patient and the patient-public interrelationships.
Aesthetic Plastic Surgery is covered in Current Contents/Clinical Medicine, SciSearch, Research Alert, Index Medicus-Medline, and Excerpta Medica/Embase.
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