Efficacy of Topical Nigella sativa Oil for Oral Wound Healing in Rats

IF 1.7 4区医学 Q2 OTORHINOLARYNGOLOGY

Laryngoscope Investigative Otolaryngology Pub Date : 2025-06-04 DOI:10.1002/lio2.70153

Alper Tabaru, Sedat Rüzgar

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引用次数: 0

Abstract

Objective

This study aimed to examine the effect of topical Nigella sativa oil (NSO) on intraoral wound healing in rats.

Materials and Methods

Forty-eight female Wistar albino rats (2.5–3 months old) were assigned to two groups (n = 24 each): A control group receiving no treatment and an NSO group receiving a topical application of 1 cc NSO. A standardized circular full-thickness wound (3 mm in diameter) was created on the mucoperiosteum of the hard palate. The animals were sacrificed on postoperative Days 3, 7, 14, and 20 to assess wound area macroscopically, wound diameter histologically, and immunohistochemically for anti–transforming growth factor-β (anti–TGF-β) and anti-vascular growth factor (anti–VGF).

Results

No significant differences were observed between groups on days 3 and 20 (p > 0.05). On Days 7 and 14, however, the NSO group exhibited significantly smaller wound areas and wound diameters (p < 0.05). Immunohistochemical staining showed lower TGF-β expression on Days 7 and 14 in the NSO group compared to control (p < 0.05), whereas VGF staining did not differ significantly (p > 0.05).

Conclusion

Topical NSO accelerated the healing of intraoral wounds, particularly by enhancing re-epithelialization between postoperative days 7 and 14. This effect appears to be mediated through TGF-β modulation and NSO's known anti-inflammatory, antimicrobial, and antioxidant actions. Topical NSO may thus be considered as an adjunct to conventional wound management in the oral cavity.

Level of Evidence

NA.

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黑皮油外用对大鼠口腔创面愈合的影响

目的研究外用黑草油对大鼠口腔创面愈合的影响。材料与方法将雌性Wistar白化大鼠48只（2.5 ~ 3月龄）分为两组（每组24只）：对照组不给予治疗，NSO组局部应用NSO 1 cc。在硬腭粘骨膜上建立一个标准的圆形全厚创面（直径3mm）。于术后第3、7、14、20天处死动物，观察创面面积、创面直径及免疫组化水平，检测抗tgf -β和抗血管生长因子水平。结果第3、20天各组间差异无统计学意义（p > 0.05）。然而，在第7天和第14天，NSO组的创面面积和创面直径明显减小（p < 0.05）。免疫组化染色显示，NSO组在第7天和第14天TGF-β表达低于对照组（p < 0.05），而VGF染色无显著差异（p < 0.05）。结论外用NSO可促进口腔内创面愈合，特别是在术后7 ~ 14天内可促进再上皮化。这种作用似乎是通过TGF-β调节和NSO已知的抗炎、抗菌和抗氧化作用介导的。因此，局部NSO可被视为口腔常规伤口处理的辅助手段。证据水平NA。

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