Metamorphosis-associated immune system maturation in Senegalese sole

IF 2.1 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

General and comparative endocrinology Pub Date : 2025-06-01 DOI:10.1016/j.ygcen.2025.114755

Sandra C. Silva , Teresa M. Correia , Manuel Manchado , Deborah M. Power

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引用次数: 0

Abstract

The thyroid hormones (THs) are proposed as putative regulators of immune system maturation in developing teleost fish. To gain insight into this process the Senegalese sole (Solea senegalensis) that has a well-characterized TH-driven metamorphosis was used. Differential gene expression analysis was performed across developmental stages (n = 3 per stage): pre-metamorphosis, onset of metamorphosis, metamorphosis, early climax, climax, and post-metamorphic juveniles. Metamorphosis is a massive gene-oriented developmental process, involving the differential expression of 8145 genes. Clustering analysis was used to identify immune-related genes with similar expression patterns to hypothalamus-pituitary-thyroid (HPT) axis-related genes. TH-regulated candidate immune genes were identified (133) and analysis of their promoter region revealed 84 contained putative TH receptor (TR) binding sites (TREs). Two consensus TRE sequences were identified in the candidate genes, 5′-ntgnGntCacan (exclusive to TRα), and 5′-nnntGgtCannn (common to both TRs). TRα-exclusive TREs were less common than those that bound interchangeably TRα and TRβ. In the promoter region, TRα-exclusive TREs were always accompanied by the pan-TRE consensus sequence, never occurring independently.

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塞内加尔比目鱼变态相关免疫系统成熟

甲状腺激素（THs）被认为是发育中的硬骨鱼免疫系统成熟的调节因子。为了深入了解这一过程，使用了具有th驱动变态特征的塞内加尔比目鱼（Solea senegalensis）。在发育阶段进行差异基因表达分析（每个阶段n = 3）：变态前、变态开始、变态、早期顶极、顶极和后变态少年。变态是一个巨大的基因导向的发育过程，涉及8145个基因的差异表达。采用聚类分析方法鉴定与HPT轴相关基因表达模式相似的免疫相关基因。鉴定了促甲状腺激素调节的候选免疫基因（133个），对其启动子区域的分析显示，84个基因含有假定的促甲状腺激素受体（TR）结合位点（TREs）。在候选基因中鉴定出两个一致的TRE序列，5 ' - ntgngntcanan （TRα独有）和5 ' -nnntGgtCannn（两个TRs共有）。与TRα和TRβ可互换结合的TREs相比，TRα-排他的TREs较少。在启动子区，tr α-独占的TREs总是伴随着pan-TRE共识序列，而不是独立发生。

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来源期刊

General and comparative endocrinology 医学-内分泌学与代谢

CiteScore

5.60

自引率

7.40%

发文量

120

审稿时长

2 months

期刊介绍： General and Comparative Endocrinology publishes articles concerned with the many complexities of vertebrate and invertebrate endocrine systems at the sub-molecular, molecular, cellular and organismal levels of analysis.