WS11.05Spatio-temporal association of Pseudomonas aeruginosa and Staphylococcus aureus in cystic fibrosis patients

IF 5.4 2区医学 Q1 RESPIRATORY SYSTEM

Journal of Cystic Fibrosis Pub Date : 2025-06-01 DOI:10.1016/j.jcf.2025.03.556

V. Waters , A. Morris , S. Eisha , Y. Yau , W. DePas , D. Nguyen , M. Gaudet

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引用次数: 0

Abstract

Objectives

Our aim was to visualize the interaction of Pseudomonas aeruginosa (PA), Staphylococcus aureus (SA) and staphylococcal protein A (SpA) in sputum of people with CF (pwCF) during pulmonary exacerbations.

Methods

This was a 3 year prospective, longitudinal observational study of pwCF with chronic PA infection from SickKids Hospital, St. Michael's Hospital and McGill University Health Centre. Sputum was collected from CF patients at baseline (B), day 0 of exacerbation (E), day 7 of antibiotic treatment (T) and recovery (R), fixed in paraformaldehyde then polymerized in a hydrogel followed by Microbial Identification after Passive CLARITY Technique (MiPACT). Ex vivo visualization of PA and SA in the sputum-hydrogels was performed by three-dimensional confocal laser scanning microscopy (CLSM) using fluorescence in situ hybridization (FISH); SpA was visualized using the protein A monoclonal antibody and concentrations measured by ELISA. PA, SA and SpA biovolume, spatial distance between microbes and number and maximum size of PA and SA aggregates were measured.

Results

Over 150 sputum samples have been collected and processed from 40 pwCF. From B to E, there was a significant increase in the number of PA aggregates in pediatric patients, while SA remained relatively constant. In addition, from B to E, a geospatial shift in PA distance to the nearest SA was observed, where PA and SA were further apart and formed discretely large aggregates at E (Day 0) compared to baseline. Patients with PA-SA co-infection had greater maximum PA aggregate size compared to those with PA mono-infection and there was a positive correlation between PA biovolume and SpA concentration measured by ELISA (p ≤ 0.0001).

Conclusion

PwCF with chronic PA infection had greater PA aggregation in their sputum that was further apart from SA aggregates during exacerbation compared to baseline. In addition, greater PA biovolume was associated with increasing concentrations of SpA.

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铜绿假单胞菌和金黄色葡萄球菌在囊性纤维化患者中的时空相关性

目的观察CF （pwCF）患者肺加重期痰液中铜绿假单胞菌（PA）、金黄色葡萄球菌（SA）和葡萄球菌蛋白A （SpA）的相互作用。方法对来自SickKids医院、St. Michael医院和McGill大学健康中心的pwCF合并慢性PA感染患者进行为期3年的前瞻性、纵向观察研究。收集CF患者在基线(B)、病情加重第0天(E)、抗生素治疗第7天(T)和康复(R)时的痰液，用多聚甲醛固定，然后用水凝胶聚合，然后用被动透明技术（MiPACT）进行微生物鉴定。利用三维共聚焦激光扫描显微镜（CLSM）和荧光原位杂交（FISH）技术对痰液水凝胶中的PA和SA进行体外观察；用蛋白A单克隆抗体可视化SpA， ELISA测定SpA浓度。测定了PA、SA和SpA的生物体积、微生物间的空间距离以及PA和SA聚集体的数量和最大粒径。结果40例pwCF患者共采集和处理了150余份痰样。从B到E，儿科患者PA聚集体数量显著增加，而SA保持相对不变。此外，从B到E，观测到PA距离最近SA的地理空间变化，其中PA和SA距离更远，与基线相比，在E（第0天）形成离散的大聚集体。PA- sa合并感染患者的最大PA聚集大小大于单纯PA感染患者，ELISA测定的PA生物体积与SpA浓度呈正相关（p≤0.0001）。结论与基线相比，慢性PA感染的pwcf患者的痰中PA聚集量更大，加重时与SA聚集物的距离更远。此外，更大的PA生物体积与增加的SpA浓度有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Cystic Fibrosis 医学-呼吸系统

CiteScore

10.10

自引率

13.50%

发文量

1361

审稿时长

50 days

期刊介绍： The Journal of Cystic Fibrosis is the official journal of the European Cystic Fibrosis Society. The journal is devoted to promoting the research and treatment of cystic fibrosis. To this end the journal publishes original scientific articles, editorials, case reports, short communications and other information relevant to cystic fibrosis. The journal also publishes news and articles concerning the activities and policies of the ECFS as well as those of other societies related the ECFS.