CRISPR/Cas Toolbox: Unveiling the secret weapon for SNP detection

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-05-31 DOI:10.1016/j.trac.2025.118321

Luwei Chai, Xinge Cui, Hongbing Liu, Yongkang Zhang, Yangwei Pan, Chenxi Li, Tao Le

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引用次数: 0

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas systems, known for its high specificity and programmability, has demonstrated significant potential in single nucleotide polymorphisms (SNPs) detection and has been extensively applied in pathogen identification, genetic screening, and tumor mutation analysis. However, the relatively low turnover rates of cis- and trans-cleavage activities limit the specificity and sensitivity of CRISPR-based SNP detection systems. In response, various CRISPR/Cas-based SNP detection strategies have been developed to achieve ultrasensitive signal responses via diverse signal amplification techniques, thereby enhancing detection resolution. This review begins by summarizing the current status and challenges of CRISPR/Cas-based SNP detection technologies, then focuses on four representative strategies for enhancing CRISPR-SNP performance. Finally, we discuss future developments that may improve the efficiency of CRISPR-SNP detection systems.

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CRISPR/Cas工具箱：揭开SNP检测的秘密武器

聚类规则间隔短回文重复序列(CRISPR)/Cas系统以其高特异性和可编程性而闻名，在单核苷酸多态性（snp）检测中显示出巨大的潜力，并已广泛应用于病原体鉴定、遗传筛选和肿瘤突变分析。然而，相对较低的顺式和反式切割活性周转率限制了基于crispr的SNP检测系统的特异性和敏感性。因此，各种基于CRISPR/ cas的SNP检测策略被开发出来，通过各种信号放大技术实现超灵敏的信号响应，从而提高检测分辨率。本文首先总结了基于CRISPR/ cas的SNP检测技术的现状和面临的挑战，然后重点介绍了提高CRISPR-SNP性能的四种具有代表性的策略。最后，我们讨论了可能提高CRISPR-SNP检测系统效率的未来发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.