Gengming Zhang, Sihan He, Xiangming He, Xueyi Gong, Jiazhen Yin, Jiusheng Li, Hongqi Zhang, Hengyi Lu, Yunjia Wang, Bo Wang
{"title":"Local Recruitment of Autologous Stem Cells via a Targeting Microgel for Precise Cartilage Repair without Surgery","authors":"Gengming Zhang, Sihan He, Xiangming He, Xueyi Gong, Jiazhen Yin, Jiusheng Li, Hongqi Zhang, Hengyi Lu, Yunjia Wang, Bo Wang","doi":"10.1002/adma.202505544","DOIUrl":null,"url":null,"abstract":"The treatment of advanced osteoarthritis (OA) remains clinically intractable due to the inability to regenerate multifocal cartilage defects, stemming from poor targeted recruitment of bone marrow-derived mesenchymal stem cells (BMSCs) and the absence of a sustained chondrogenic microenvironment at the injury sites. In this work, an antibody-mediated gelatin methacrylate-based hydrogel microsphere modified by TGFβ-affinity peptides (TRG microsphere) is developed, to precisely target and repair scattered cartilage injuries by only intraarticular injection without any surgical assistance. By leveraging the specific expression of type I collagen in OA cartilage lesions, the type I collagen antibodies anchoring on TRG's surface enable the specific and accurate targeting of the multiple injury areas that need regeneration. In the meantime, the TGFβ-affinity peptides incorporated in the TRG microsphere can capture the endogenous TGFβ, a growth factor that can recruit BMSCs and promote their differentiation, to precisely induce the hyaline-like cartilage regeneration locally. In a rat model of advanced OA, a single intra-articular injection of TRG microspheres can repair scattered cartilage defects, restore glycosaminoglycan deposition, and alleviate joint dysfunction. This study proposes an injection-based strategy that enables continuous recruitment of endogenous BMSCs for precise cartilage regeneration, eliminating complex invasive procedures and patient discomfort.","PeriodicalId":114,"journal":{"name":"Advanced Materials","volume":"19 1","pages":""},"PeriodicalIF":27.4000,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Materials","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1002/adma.202505544","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
The treatment of advanced osteoarthritis (OA) remains clinically intractable due to the inability to regenerate multifocal cartilage defects, stemming from poor targeted recruitment of bone marrow-derived mesenchymal stem cells (BMSCs) and the absence of a sustained chondrogenic microenvironment at the injury sites. In this work, an antibody-mediated gelatin methacrylate-based hydrogel microsphere modified by TGFβ-affinity peptides (TRG microsphere) is developed, to precisely target and repair scattered cartilage injuries by only intraarticular injection without any surgical assistance. By leveraging the specific expression of type I collagen in OA cartilage lesions, the type I collagen antibodies anchoring on TRG's surface enable the specific and accurate targeting of the multiple injury areas that need regeneration. In the meantime, the TGFβ-affinity peptides incorporated in the TRG microsphere can capture the endogenous TGFβ, a growth factor that can recruit BMSCs and promote their differentiation, to precisely induce the hyaline-like cartilage regeneration locally. In a rat model of advanced OA, a single intra-articular injection of TRG microspheres can repair scattered cartilage defects, restore glycosaminoglycan deposition, and alleviate joint dysfunction. This study proposes an injection-based strategy that enables continuous recruitment of endogenous BMSCs for precise cartilage regeneration, eliminating complex invasive procedures and patient discomfort.
期刊介绍:
Advanced Materials, one of the world's most prestigious journals and the foundation of the Advanced portfolio, is the home of choice for best-in-class materials science for more than 30 years. Following this fast-growing and interdisciplinary field, we are considering and publishing the most important discoveries on any and all materials from materials scientists, chemists, physicists, engineers as well as health and life scientists and bringing you the latest results and trends in modern materials-related research every week.