Christopher Castillo, Su-Ji Jeon, Khoi Nguyen L. Hoang, Claire Alford, Erica Svendahl, Chaoyi Deng, Yi Wang, Yinhan Wang, Xingfei Wei, Rigoberto Hernandez, Jason C. White, Korin E. Wheeler, Catherine J. Murphy, Juan Pablo Giraldo
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引用次数: 0
Abstract
The impact of nanomaterial transformations on photosynthetic proteins remains largely unknown. We report positively charged iron oxide (Fe3O4) nanoparticles experience transformations in Arabidopsis thaliana plants in vivo that alter the formation and function of RuBisCO protein corona, a key carbon fixation enzyme. In vitro, negatively charged Fe3O4 nanoparticles impact the RuBisCO function but not their positively charged counterparts. Computational and in vitro proteomic analyses revealed that positively charged Fe3O4 nanoparticles preferentially bind to a RuBisCO small subunit that lacks active carboxylation sites. However, both positively and negatively charged nanoparticles decrease RuBisCO carboxylation activity after experiencing transformations in vivo by 3.0 and 1.7 times relative to the controls, respectively. The pH- and lipid-coating-dependent transformations that occur during nanoparticle transport across plant membranes enhance RuBisCO binding to positively charged nanoparticles affecting its distribution in chloroplasts. Elucidating the rules of how nanoparticle properties and transformations affect photosynthetic coronas is crucial for sustainable nano-enabled agriculture.
期刊介绍:
Nature Nanotechnology is a prestigious journal that publishes high-quality papers in various areas of nanoscience and nanotechnology. The journal focuses on the design, characterization, and production of structures, devices, and systems that manipulate and control materials at atomic, molecular, and macromolecular scales. It encompasses both bottom-up and top-down approaches, as well as their combinations.
Furthermore, Nature Nanotechnology fosters the exchange of ideas among researchers from diverse disciplines such as chemistry, physics, material science, biomedical research, engineering, and more. It promotes collaboration at the forefront of this multidisciplinary field. The journal covers a wide range of topics, from fundamental research in physics, chemistry, and biology, including computational work and simulations, to the development of innovative devices and technologies for various industrial sectors such as information technology, medicine, manufacturing, high-performance materials, energy, and environmental technologies. It includes coverage of organic, inorganic, and hybrid materials.