In vivo transformations of positively charged nanoparticles alter the formation and function of RuBisCO photosynthetic protein corona

IF 38.1 1区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Christopher Castillo, Su-Ji Jeon, Khoi Nguyen L. Hoang, Claire Alford, Erica Svendahl, Chaoyi Deng, Yi Wang, Yinhan Wang, Xingfei Wei, Rigoberto Hernandez, Jason C. White, Korin E. Wheeler, Catherine J. Murphy, Juan Pablo Giraldo
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Abstract

The impact of nanomaterial transformations on photosynthetic proteins remains largely unknown. We report positively charged iron oxide (Fe3O4) nanoparticles experience transformations in Arabidopsis thaliana plants in vivo that alter the formation and function of RuBisCO protein corona, a key carbon fixation enzyme. In vitro, negatively charged Fe3O4 nanoparticles impact the RuBisCO function but not their positively charged counterparts. Computational and in vitro proteomic analyses revealed that positively charged Fe3O4 nanoparticles preferentially bind to a RuBisCO small subunit that lacks active carboxylation sites. However, both positively and negatively charged nanoparticles decrease RuBisCO carboxylation activity after experiencing transformations in vivo by 3.0 and 1.7 times relative to the controls, respectively. The pH- and lipid-coating-dependent transformations that occur during nanoparticle transport across plant membranes enhance RuBisCO binding to positively charged nanoparticles affecting its distribution in chloroplasts. Elucidating the rules of how nanoparticle properties and transformations affect photosynthetic coronas is crucial for sustainable nano-enabled agriculture.

Abstract Image

带正电的纳米颗粒在体内的转化改变了RuBisCO光合蛋白冠的形成和功能
纳米材料转化对光合蛋白的影响在很大程度上仍然未知。我们报道了带正电荷的氧化铁(Fe3O4)纳米颗粒在拟南芥植物体内经历转化,改变RuBisCO蛋白冠的形成和功能,这是一种关键的碳固定酶。在体外,带负电的Fe3O4纳米颗粒会影响RuBisCO的功能,而带正电的Fe3O4纳米颗粒则不会。计算和体外蛋白质组学分析表明,带正电荷的Fe3O4纳米颗粒优先结合缺乏活性羧化位点的RuBisCO小亚基。然而,带正电和带负电的纳米颗粒在体内经历转化后,RuBisCO羧化活性分别比对照降低了3.0倍和1.7倍。纳米颗粒在植物膜上运输过程中发生的pH和脂质涂层依赖性转化增强了RuBisCO与带正电的纳米颗粒的结合,影响了其在叶绿体中的分布。阐明纳米粒子特性和转化如何影响光合日冕的规则对于可持续的纳米农业至关重要。
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来源期刊
Nature nanotechnology
Nature nanotechnology 工程技术-材料科学:综合
CiteScore
59.70
自引率
0.80%
发文量
196
审稿时长
4-8 weeks
期刊介绍: Nature Nanotechnology is a prestigious journal that publishes high-quality papers in various areas of nanoscience and nanotechnology. The journal focuses on the design, characterization, and production of structures, devices, and systems that manipulate and control materials at atomic, molecular, and macromolecular scales. It encompasses both bottom-up and top-down approaches, as well as their combinations. Furthermore, Nature Nanotechnology fosters the exchange of ideas among researchers from diverse disciplines such as chemistry, physics, material science, biomedical research, engineering, and more. It promotes collaboration at the forefront of this multidisciplinary field. The journal covers a wide range of topics, from fundamental research in physics, chemistry, and biology, including computational work and simulations, to the development of innovative devices and technologies for various industrial sectors such as information technology, medicine, manufacturing, high-performance materials, energy, and environmental technologies. It includes coverage of organic, inorganic, and hybrid materials.
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