{"title":"Molecular Mechanisms of Intervertebral Disc Degeneration: Significance of SPARC Gene Expression.","authors":"Jiancong Zeng, Song Yan, Wei Dong, Jinsong Wei","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To explore the role of the SPARC gene in intervertebral disc degeneration (IDD) using bioinformatics analysis combined with <i>in vitro</i> experiments.</p><p><strong>Methods: </strong>Single-cell sequencing data from the GEO and CNGBdb databases were analyzed to identify differential SPARC expression across degeneration grades. <i>In vitro</i>, nucleus pulposus cells were treated with LPS and SPARC, followed by RT-qPCR, Western blot, immunofluorescence, and immunohistochemistry to assess gene and protein expression changes.</p><p><strong>Results: </strong>SPARC expression was significantly upregulated in degenerated nucleus pulposus cells and correlated with altered COL2A1 and ACAN expression.</p><p><strong>Conclusion: </strong>SPARC may play a key role in IDD pathogenesis, offering a potential therapeutic target for future interventions. These findings contribute to our understanding of the molecular mechanisms underlying IDD and pave the way for targeted therapies.</p>","PeriodicalId":16430,"journal":{"name":"Journal of musculoskeletal & neuronal interactions","volume":"25 2","pages":"239-247"},"PeriodicalIF":1.7000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12131075/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of musculoskeletal & neuronal interactions","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Objectives: To explore the role of the SPARC gene in intervertebral disc degeneration (IDD) using bioinformatics analysis combined with in vitro experiments.
Methods: Single-cell sequencing data from the GEO and CNGBdb databases were analyzed to identify differential SPARC expression across degeneration grades. In vitro, nucleus pulposus cells were treated with LPS and SPARC, followed by RT-qPCR, Western blot, immunofluorescence, and immunohistochemistry to assess gene and protein expression changes.
Results: SPARC expression was significantly upregulated in degenerated nucleus pulposus cells and correlated with altered COL2A1 and ACAN expression.
Conclusion: SPARC may play a key role in IDD pathogenesis, offering a potential therapeutic target for future interventions. These findings contribute to our understanding of the molecular mechanisms underlying IDD and pave the way for targeted therapies.
期刊介绍：
The Journal of Musculoskeletal and Neuronal Interactions (JMNI) is an academic journal dealing with the pathophysiology and treatment of musculoskeletal disorders. It is published quarterly (months of issue March, June, September, December). Its purpose is to publish original, peer-reviewed papers of research and clinical experience in all areas of the musculoskeletal system and its interactions with the nervous system, especially metabolic bone diseases, with particular emphasis on osteoporosis. Additionally, JMNI publishes the Abstracts from the biannual meetings of the International Society of Musculoskeletal and Neuronal Interactions, and hosts Abstracts of other meetings on topics related to the aims and scope of JMNI.
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