First Report of Ganoderma ryvardenii causing Basal Stem Rot (BSR) disease on oil palm ( Elaeis guineensis Jacq.) in Ghana.

Q2 Pharmacology, Toxicology and Pharmaceutics

F1000Research Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI:10.12688/f1000research.161972.2

Emmanuellah Lekete-Lawson, Grace C van der Puije, Enoch A Osekre, Frank K Ackah

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引用次数: 0

Abstract

Backgrounds: Oil palm ( Elaeis guineensis Jacq.), is the most significant and highest-yielding crop among oil-producing crops worldwide. In 2020/2022, Basal stem rot (BSR) disease was observed in six oil palm growing Districts in Ghana.

Methods: Field study and laboratory analysis were conducted. A random sampling technique was used to select five plantation blocks from each District. Single-point disease assessments were done using Standard Operating Procedure (SOP) with a severity scale of 0-4. Molecular assays were performed on each sample using nucleic acid as a template. ITS and GanET sequence analysis were performed along with the formation of a phylogenetic tree using the FASTA algorithm with the Fungus database from EBI and NCBI GenBank. Koch's postulate was followed to confirm the disease.

Results: The disease incidence was 11.3 % with the highest severity score of 4. Basal Stem Rot (BSR) is characterised by progressive stem decay coupled with the formation of large, perennial, woody basidiocarps. The average measurement of 2-65 cm in diameter on infected palms. Culture colonies were white, striated, undulating, woolly-cottony, and creamish pigment on the reverse, depicting attributes of Ganoderma fungus. Molecular confirmation was done by combining the ITS sequence of top matches of >97% to members of the genus Ganoderma, >98% and 99.3% identity to three sequences of Ganoderma sp. (HM138671; HM138670 and HM138672) generated from strains assigned to Ganoderma ryvardenii and compared with 132 published sequences of Ganoderma isolates.

Conclusion: This study presents the first report of Ganoderma ryvardenii causing BSR disease on oil palm in Ghana, potentially the first in West Africa, and second in Africa. Notably, the pathogen was previously first reported to cause similar disease on oil palm in Cameroon, highlighting its emerging threat to oil palm production in the Sub-Saharan African region.

查看原文本刊更多论文

加纳油棕（Elaeis guineensis Jacq.）基底茎腐病（BSR）的灵芝首次报道。

背景：油棕（Elaeis guineensis Jacq.）是世界上最重要和产量最高的油料作物。2020/2022年，在加纳六个油棕种植区发现了基底茎腐病（BSR）。方法：实地调查和实验室分析。采用随机抽样的方法，从每个区选取5个种植小区。采用标准操作程序（SOP）进行单点疾病评估，严重程度评分为0-4。以核酸为模板对每个样品进行分子分析。利用EBI和NCBI GenBank的真菌数据库进行ITS和GanET序列分析，并采用FASTA算法构建系统发育树。科赫的假设被用来证实这种疾病。结果：本病发病率为11.3%，严重程度评分最高为4分。BSR的特征是茎腐烂，大多年生，木质托架担子果，平均直径为2-65厘米。培养菌落为白色，条纹状，波浪形，绵质状，背面为乳白色，描绘了灵芝真菌的特征。利用ITS序列对Ganoderma属成员的最高匹配度>97%、Ganoderma sp. (HM138671；HM138670和HM138672)，并与已发表的132个灵芝菌株序列进行比较。结论：这是在加纳首次报道由灵芝引起油棕BSR病，在非洲可能是第二次报道。然而，该病原体首次在喀麦隆的油棕中引起类似疾病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

F1000Research Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

5.00

自引率

0.00%

发文量

1646

审稿时长

1 weeks

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