Species diversity, virulence, and antimicrobial resistance of the nasal staphylococcal and mammaliicoccal biota of reindeer.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2025-06-02 DOI:10.1186/s12917-025-04680-5

Jolanta Kochel-Karakulska, Marta Maślanko, Marta Woroszyło, Małgorzata Szewczuk, Bartłomiej Grygorcewicz, Karol Fijałkowski

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引用次数: 0

Abstract

Background: Staphylococcus (S.) spp. and Mammaliicoccus (M.) spp., in addition to their established role as components of the human and animal microbiota, can also cause opportunistic infections. This study aimed to characterize bacteria recovered from nasal cavities of healthy adult reindeer from two farms located in Poland (15 reindeer) and Germany (15 reindeer). The research include bacteria isolation, species identification, detection of selected superantigen (SAg) genes, assessment of biofilm-forming capability in vitro, and evaluation of antimicrobial resistance.

Results: Seventy-four staphylococci and mammaliicocci from 14 different species were isolated from 30 nasal swabs, with one to four strains obtained from each reindeer. The most frequently identified species was S. equorum, followed by S. succinus, M. sciuri, S. xylosus, M. lentus, S. chromogenes, S. devriesei, M. vitulinus, S. auricularis, S. agnetis, S. edaphicus, S. petrasii, S. simulans, and S. warneri. A greater species diversity was observed among the reindeer from Poland compared to those from Germany. All isolated bacteria were coagulase negative and clumping factor negative and did not carry any of the 21 analyzed SAg genes. M. sciuri demonstrated the highest antimicrobial resistance (100%), followed by S. succinus (91%) and S. equorum (78%). Resistance to rifampicin was the most common (30% strains). Sixteen strains (22%) exhibited biofilm production at least 10% greater than the strong biofilm-forming S. aureus ATCC 6538.

Conclusions: This study reveals a significant knowledge gap regarding the nasal microbiota of reindeer. It contributes to our understanding of staphylococcal and mammaliicoccal biota of reindeer and underscores the necessity for monitoring of microbial populations to assess their health implications for both animals and humans, particularly concerning the zoonotic transmission of bacteria.

查看原文本刊更多论文

驯鹿鼻葡萄球菌和哺乳动物球菌生物群的物种多样性、毒力和抗菌素耐药性。

背景：葡萄球菌（S.）和哺乳球菌（M.）除了作为人类和动物微生物群组成部分的既定作用外，还可引起机会性感染。本研究旨在对来自波兰（15头驯鹿）和德国（15头驯鹿）两个农场的健康成年驯鹿鼻腔中恢复的细菌进行特征分析。研究内容包括细菌分离、菌种鉴定、筛选超抗原（SAg）基因检测、体外生物膜形成能力评估、抗菌药物耐药性评估等。结果：从30只驯鹿鼻拭子中分离出14种葡萄球菌和哺乳动物球菌74株，每只驯鹿分离出1 ~ 4株。最常被鉴定的种为equorum，其次为succinus、M. sciuri、S. xylosus、M. lentus、S. chromogenes、S. devriesei、M. vitulinus、S. auricularis、S. agnetis、S. edaphicus、S. petrasii、S. simulans和S. warneri。与德国驯鹿相比，波兰驯鹿的物种多样性更大。所有分离的细菌均为凝固酶阴性和凝块因子阴性，不携带21个分析的SAg基因中的任何一个。耐药程度最高的是M. sciuri(100%)，其次是S. succinus (91%), equorum（78%）。利福平耐药最为常见（30%菌株）。16株菌株（22%）的生物膜产量比强生物膜形成金黄色葡萄球菌ATCC 6538高出至少10%。结论：本研究揭示了关于驯鹿鼻腔微生物群的重要知识差距。它有助于我们了解驯鹿的葡萄球菌和哺乳动物球菌生物群，并强调了监测微生物种群的必要性，以评估它们对动物和人类的健康影响，特别是关于细菌的人畜共患传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.