Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis.

IF 1.5 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-06-02 DOI:10.1007/s11686-025-01045-8

Zeinab R Hassan, Eman M Mahmoud, Yasmeen M Shaaban, Shiamaa Zakaria Elshora, Doaa E A Salama, Ranya M Abdelgalil, Mona T Koullah, Nora Seliem, Marwa H Sedira, Shimaa A Mohamed, Mai Ali Mohammad Etewa, Sara Nagdy Mahmoud Mousa, Ahmed Shaban Abdelmonsef Abdelmaksoud, Shimaa Attia Atta, Marwa Zakaria, Shimaa R Emam, M Hassan, Galal Khamis

{"title":"Ameliorative Effects of Blue LED Irradiated and Non-Irradiated Fenugreek Seed Extracts on Murine Trichinosis.","authors":"Zeinab R Hassan, Eman M Mahmoud, Yasmeen M Shaaban, Shiamaa Zakaria Elshora, Doaa E A Salama, Ranya M Abdelgalil, Mona T Koullah, Nora Seliem, Marwa H Sedira, Shimaa A Mohamed, Mai Ali Mohammad Etewa, Sara Nagdy Mahmoud Mousa, Ahmed Shaban Abdelmonsef Abdelmaksoud, Shimaa Attia Atta, Marwa Zakaria, Shimaa R Emam, M Hassan, Galal Khamis","doi":"10.1007/s11686-025-01045-8","DOIUrl":null,"url":null,"abstract":"Background: Trichinosis is a severe parasitic disease with a wide distribution and potential to affect humans. Available chemotherapeutic agents exhibit limited efficacy and are associated with numerous side effects. This study evaluated the ameliorative effects of blue LED irradiated and non-irradiated fenugreek seed extracts on experimental trichinosis.Methods: Eighty-four mice were divided into seven groups; each further subdivided into intestinal and muscular phases (six mice per subgroup): non-infected non-treated controls, infected non-treated controls, infected albendazole-treated, infected non-irradiated fenugreek extract-treated, infected blue LED irradiated fenugreek extract-treated, infected non-irradiated fenugreek and albendazole-treated, and infected blue LED irradiated fenugreek and albendazole-treated. Mice were sacrificed on the 7th-day post-infection for the intestinal phase and the 40th day for the muscular phase. Small intestine, muscle tissues, and serum samples were collected to assess parasitic load, histopathological changes, TGF-β immunohistochemical expression, serum IFN-γ levels, oxidative stress markers (MDA, nitrate/SOD, and catalase), and BAX gene expression as an apoptotic marker. Metabolomic profiling of extract was pursued to spot differential expression of metabolites.Results: Study outcomes demonstrated that blue LED irradiated and non-irradiated fenugreek seed extracts combined with albendazole exhibited superior efficacy in reducing adult and larval burdens, improving pathological changes, decreasing IFN-γ levels, mitigating oxidative stress (reduced MDA and nitrate along with elevated SOD and catalase), and downregulating BAX expression. The observed metabolic differences were primarily driven by the upregulation of steroids, downregulation of most alkaloids, and dysregulation various flavonoids in the irradiated extract.Conclusion: Blue LED irradiated and non-irradiated fenugreek seed extracts can enhance albendazole's activity against trichinosis.","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 3","pages":"119"},"PeriodicalIF":1.5000,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12130083/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11686-025-01045-8","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Trichinosis is a severe parasitic disease with a wide distribution and potential to affect humans. Available chemotherapeutic agents exhibit limited efficacy and are associated with numerous side effects. This study evaluated the ameliorative effects of blue LED irradiated and non-irradiated fenugreek seed extracts on experimental trichinosis.

Methods: Eighty-four mice were divided into seven groups; each further subdivided into intestinal and muscular phases (six mice per subgroup): non-infected non-treated controls, infected non-treated controls, infected albendazole-treated, infected non-irradiated fenugreek extract-treated, infected blue LED irradiated fenugreek extract-treated, infected non-irradiated fenugreek and albendazole-treated, and infected blue LED irradiated fenugreek and albendazole-treated. Mice were sacrificed on the 7th-day post-infection for the intestinal phase and the 40th day for the muscular phase. Small intestine, muscle tissues, and serum samples were collected to assess parasitic load, histopathological changes, TGF-β immunohistochemical expression, serum IFN-γ levels, oxidative stress markers (MDA, nitrate/SOD, and catalase), and BAX gene expression as an apoptotic marker. Metabolomic profiling of extract was pursued to spot differential expression of metabolites.

Results: Study outcomes demonstrated that blue LED irradiated and non-irradiated fenugreek seed extracts combined with albendazole exhibited superior efficacy in reducing adult and larval burdens, improving pathological changes, decreasing IFN-γ levels, mitigating oxidative stress (reduced MDA and nitrate along with elevated SOD and catalase), and downregulating BAX expression. The observed metabolic differences were primarily driven by the upregulation of steroids, downregulation of most alkaloids, and dysregulation various flavonoids in the irradiated extract.

Conclusion: Blue LED irradiated and non-irradiated fenugreek seed extracts can enhance albendazole's activity against trichinosis.

查看原文本刊更多论文

蓝色LED照射和未照射葫芦巴籽提取物对小鼠旋毛虫病的改善作用。

背景：旋毛虫病是一种严重的寄生虫病，分布广泛，有可能影响人类。现有的化疗药物表现出有限的疗效，并伴有许多副作用。本研究评价了蓝色LED照射和未照射葫芦巴籽提取物对实验性旋毛虫病的改善作用。方法：84只小鼠分为7组；每个亚组进一步细分为肠道和肌肉阶段（每亚组6只小鼠）：未感染的非治疗对照组、感染的非治疗对照组、感染的阿苯达唑治疗组、感染的非辐照葫芦巴提取物治疗组、感染的非辐照葫芦巴提取物治疗组、感染的非辐照葫芦巴和阿苯达唑治疗组、感染的蓝色LED照射葫芦巴和阿苯达唑治疗组。感染后第7天处死小鼠为肠道期，第40天处死小鼠为肌肉期。收集小肠、肌肉组织和血清样本，评估寄生虫负荷、组织病理学变化、TGF-β免疫组织化学表达、血清IFN-γ水平、氧化应激标志物（MDA、硝酸盐/SOD和过氧化氢酶）和BAX基因表达作为凋亡标志物。对提取物进行代谢组学分析，以发现代谢物的差异表达。结果：研究结果表明，蓝色LED照射和未照射的胡芦巴籽提取物联合阿苯达唑在减轻成虫和幼虫负担、改善病理变化、降低IFN-γ水平、减轻氧化应激（降低MDA和硝酸盐，升高SOD和过氧化氢酶）和下调BAX表达方面均具有显著效果。所观察到的代谢差异主要是由辐照提取物中类固醇的上调、大多数生物碱的下调和各种黄酮类化合物的失调所驱动的。结论：蓝色LED辐照和非辐照葫芦巴籽提取物均能增强阿苯达唑抗旋毛虫病的活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.