Depth understanding on the pollutant dispersion mechanism in large scale industrial buildings is significant for improving the air quality and supplying technique references on ventilation system design. Although a large amount of researches on ventilation system have been conducted, the effect of the intermittent ventilation mode on the pollutant diffusion with multiple release sources is still unclear. In the present study, the dispersion performance of hydrogen sulfide in an actual rubber processing plant was comprehensively investigated by model test and numerical simulation. The hybrid natural ventilation with mechanical exhaust was designed and used for the removal of hydrogen sulfide. A model test rig was established to measure the pollutant distribution in a transparent glass chamber with substitutive fluid sulfur hexafluoride. The numerical model of pollutant dispersion was built with considerations of temperature stratification and concentration diffusion. Compared against the test data, the prediction deviation of the numerical model was limited within 10%. The influence of the intermittent ventilation modes on the hydrogen sulfide dispersion was investigated and analyzed in detail. Some findings were obtained finally. This study could enrich the ventilation design on hydrogen sulfide dispersion in actual industrial plants and may supply some new insights on the dispersion mechanism of pollutants.