First Report of Botryosphaeria dothidea Causing Leaf Blight on Aesculus chinensis in China

IF 1.1 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2025-06-02 DOI:10.1111/jph.70085

Cuicui Wang, Lina Liu, Yongguang Liu, Zihao Wu, Chao Li, Haoqin Pan

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引用次数: 0

Abstract

In July 2024, a leaf blight disease was observed on Aesculus chinensis plants in Shandong Province, China. The disease presented as brown lesions progressing to light brown. The morphological features of colonies and conidia observed on PDA medium were consistent with those of Botryosphaeria dothidea. Pathogenicity tests were conducted on A. chinensis leaves using both greenhouse-grown seedlings and field-grown mature trees through a wound inoculation method. Brown lesions developed on the inoculated leaves of both seedlings and mature trees, whereas no symptoms appeared on the control leaves. The ITS, translation elongation factor 1-alpha (TEF1) and β-tubulin (TUB) regions from the isolates were amplified and sequenced. BLAST analysis of these three genes revealed 99.61%–100.00% identity with the corresponding sequences of B. dothidea ex-epitype strain (CBS 115476) available in GenBank. Phylogenetic analysis further confirmed the identification of the pathogen as B. dothidea. This study represents the first report of B. dothidea causing leaf blight disease on A. chinensis in China.

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引起中国七叶树叶枯病的北方葡萄球孢菌首次报道

2024年7月，对山东省七叶树（Aesculus chinensis）植物进行了叶枯病观察。疾病表现为棕色病变进展为浅棕色。在PDA培养基上观察到的菌落和分生孢子的形态特征与斑点球孢的形态特征一致。采用伤口接种法，对温室栽培和大田成熟乔木叶片进行了致病性试验。接种过的幼苗和成熟树木的叶片上都出现了棕色病变，而对照叶片上没有出现任何症状。对分离菌株的ITS、翻译延伸因子1- α (TEF1)和β-微管蛋白（TUB）区域进行扩增和测序。BLAST分析结果显示，这3个基因与GenBank中的dothidea原表型菌株（CBS 115476）对应序列的同源性为99.61% ~ 100.00%。系统发育分析进一步证实该病原菌为dothidea。本研究是国内首次报道白桦叶枯病的病原菌。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.