Molecular characterization, phylogenetic analysis and histopathological changes of Eimeria species infecting domestic pigeons (Columba livia domestica) in Giza governorate, Egypt

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-05-29 DOI:10.1016/j.vprsr.2025.101290

Fatema A. Gamal , Azza M. Abdel-Wahab , Marwa M. Khalifa , Marwa S. Khattab , Ahmed M. Nassar

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Abstract

The study was aimed to perform molecular identification of Eimeria spp. infecting domestic pigeons in Giza governorate, Egypt. Also, to study its life cycle in experimentally infected squabs. Molecular identification revealed five species of Eimeria (E. labbeana, E.columbarum, E.columbae, E.columbinae and E. chalcoptereae). Molecular characterization using two primers, the PCR products of Eimeria spp. revealed that the amplified products of the 18S rRNA region had ∼400 bp and 500 bp. Sequencing and phylogenetic analysis of the PCR results indicated that the species under investigation were E. labbeana, E.columbarum, E.columbae, E.columbinae and E. chalcoptereae under accession numbers OR420916, OR554388, OR562036, OR420908 and OR553660, respectively. Experimental infection of squabs with mixed Eimeria spp. revealed that trophozoites, schizonts, macro, microgametocytes and immature oocysts appeared at 12–36 h, 3–4 days, 4–5 days and 6 days post infection (PI), respectively. Histopathological changes in different intestinal parts showed sloughed epithelium, shortened villi and leukocytic cells infiltration. This study is considered as the first molecular characterization and phylogenetic analysis of Eimeria spp. infecting domestic pigeons in Egypt.

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埃及吉萨省家鸽感染艾美耳球虫的分子特征、系统发育分析及组织病理学变化

本研究旨在对感染埃及吉萨省家鸽的艾美耳球虫进行分子鉴定。同时，在实验感染的乳鸽中研究其生命周期。分子鉴定发现5种艾美耳球虫，分别为labbeana艾美耳球虫、columbarum艾美耳球虫、columbae艾美耳球虫、columbinae艾美耳球虫和chalcoptereae艾美耳球虫。利用两种引物对艾美耳球虫的PCR产物进行分子鉴定，扩增产物的18S rRNA区长度分别为~ 400 bp和500 bp。测序和系统发育分析结果表明，该菌株分别为E. labbeana、E.columbarum、E.columbae、E.columbinae和E. chalcoptereae，登记号分别为OR420916、OR554388、OR562036、OR420908和OR553660。实验结果表明，感染后12-36 h、3-4 d、4-5 d和6 d分别出现滋养体、分裂体、大配子体、小配子体和未成熟卵囊。肠各部位组织病理变化表现为上皮脱落，绒毛缩短，白细胞浸润。本研究首次对埃及家鸽感染艾美耳球虫进行了分子鉴定和系统发育分析。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).