The extracts of small-leaved kudingcha as potential modulators of β-cells for the therapy of type 2 diabetes mellitus

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-06-02 DOI:10.1016/j.jff.2025.106922

MingJuan Chen , Lirong Huang , Qinqin Xiong , Ningning Zan , Zhi Wang , Yongfen Bai , Jia Yu , Sha Cheng , Hong Zeng , Mashaal Ahmad , Heng Luo

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Abstract

Small-leaved Kudingcha (SLK), a unique variety of tea, has blood sugar-lowering effect. However, the underlying molecular mechanisms of lowering blood sugar is still unclear. Here, the crude extract of SLK and its four fractions of petroleum ether (SLK-PE), ethyl acetate (SLK-EA), n-butanol (SLK-NA), and water (SLK-WA) were prepared and analyzed by Liquid Chromatography-Mass spectrometry/Mass spectrometry. The findings revealed that SLK-PE and SLK-NA ameliorated polydipsia, polyphagia, and polyuria in T2DM mice, as well as serum biochemical indicators. We found that SLK-PE and SLK-NA could enhance blood sugar regulation ability and improve the degree of islet cells damage by upregulating the relative protein levels of p-PI3K/PI3K and p-AKT/AKT ratios, as well as Glut4, Nrf2, INS, SIRT1 and PGC-1α, while downregulating the expression levels of Keap-1, FoXO1, GSK3β, and GCG. Cellular experiment results further demonstrated that SLK-PE and SLK-NA could promote MIN6 cells proliferation and increasing insulin secretion via PI3K/AKT signaling pathway. Additionally, SLK-PE and SLK-NA have a high level of safety. The findings demonstrate that SLK-PE and SLK-NA could serve as potential functional additives for the prophylaxis and management of diabetes and linked metabolic conditions.

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苦丁茶提取物作为β-细胞的潜在调节剂治疗2型糖尿病

小叶苦丁茶（SLK）是一种独特的茶叶品种，具有降血糖作用。然而，降低血糖的潜在分子机制仍不清楚。采用液相色谱-质谱联用/质谱联用的方法，制备了SLK粗提物及其石油醚（SLK- pe）、乙酸乙酯（SLK- ea）、正丁醇（SLK- na）和水（SLK- wa）四个组分。结果显示，SLK-PE和SLK-NA可改善T2DM小鼠的多饮、多食和多尿，并改善血清生化指标。我们发现，SLK-PE和SLK-NA通过上调p-PI3K/PI3K和p-AKT/AKT比值以及Glut4、Nrf2、INS、SIRT1和PGC-1α的相对蛋白水平，下调Keap-1、fox01、GSK3β和GCG的表达水平，增强血糖调节能力，改善胰岛细胞损伤程度。细胞实验结果进一步表明，SLK-PE和SLK-NA可通过PI3K/AKT信号通路促进MIN6细胞增殖，增加胰岛素分泌。此外，SLK-PE和SLK-NA具有较高的安全性。研究结果表明，SLK-PE和SLK-NA可作为潜在的功能添加剂，用于预防和管理糖尿病及相关代谢疾病。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.