The association between XRCC2 and BCORL1 expression with sperm DNA fragmentation index in infertile men candidates undergoing intracytoplasmic sperm injection (ICSI)

IF 2.5 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2025-05-18 DOI:10.1016/j.tice.2025.102982

Reza Paknejad , Farangis Ghassemi , Masumeh Golestan Jahromi , Samaneh Zolghadri

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引用次数: 0

Abstract

Introduction

This study aims to investigate the relative gene expression of XRCC2 and BCORL1 in the sperm and its association with DNA fragmentation index (DFI) and sperm parameters in infertile men candidate for fertilization by intracytoplasmic sperm injection (ICSI).

Methods

The semen samples from the infertile men candidates were centrifuged using the gradient method to obtain pure sperm. DFI of samples was measured using the sperm chromatin dispersion (SCD) assay. The samples were divided into three groups based on the DFI (with DFI ≤ 15 % (excellent fertility potential), DFI= 15–30 % (good fertility potential), and DFI > 30 % (fair fertility potential). Then, according to WHO criteria, sperm parameters were evaluated. Finally, the expression of XRCC2 and BCORL1 was measured using real-time PCR.

Results

This study showed that the expression of XRCC2 in groups with DFI > 30 % was significantly decreased compared to the groups with DFI ≤ 15 % and DFI= 15–30 % (P < 0.05). Furthermore, according to the correlation coefficients data, the mRNA expression level of XRCC2 was correlated positively with fertilization rate and negatively with DFI (p < 0.05). However, there was no association between BCORL1 expression with DFI and other sperm parameters.

Conclusion

This investigation revealed that lowering of XRCC2 expression raises sperm DNA damage.

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不育男性接受胞浆内单精子注射（ICSI）后XRCC2和BCORL1表达与精子DNA断裂指数的关系

本研究旨在探讨精子内单精子注射（ICSI）不育男性精子中XRCC2和BCORL1基因的相对表达及其与DNA片段化指数（DFI）和精子参数的关系。方法采用梯度法对不育候选者的精液进行离心，获得纯精子。用精子染色质分散（SCD）法测定样品的DFI。根据DFI (DFI≤15 %（优秀生育潜力）、DFI= 15 - 30 %（良好生育潜力）和DFI >； 30 %（一般生育潜力）将样本分为三组。然后，根据WHO标准，评估精子参数。最后，采用实时荧光定量PCR检测XRCC2和BCORL1的表达。结果本研究显示，与DFI≤ 15 %和DFI= 15 - 30 %组相比，DFI= 30 %组XRCC2的表达显著降低（P <； 0.05）。从相关系数数据看，XRCC2 mRNA表达量与受精率呈正相关，与DFI呈负相关（p <； 0.05）。然而，BCORL1的表达与DFI和其他精子参数之间没有关联。结论降低XRCC2表达可增加精子DNA损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.