Identification of Differential Fibrocartilage Degradation Between Sexes in a Burn-Synovectomy Temporomandibular Osteoarthritis Mouse Model.

IF 1.7 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Orthodontics & Craniofacial Research Pub Date : 2025-05-31 DOI:10.1111/ocr.12946

Amy Z Lu, Jeffrey Nickel, Ginny Ching-Yun Hsu

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Abstract

Background: Temporomandibular joint osteoarthritis is a degenerative disease with a higher prevalence in women, yet its sex-specific mechanisms remain unclear. This study investigates fibrocartilage degradation in a burn-synovectomy TMJ OA mouse model, hypothesizing that female mice exhibit greater cartilage degradation due to differential inflammatory responses.

Methods: PRG4-CreERT2; Ai9 mice (12-week-old males and females) underwent burn-synovectomy injuries and were euthanized at 1, 3, and 9 weeks for histological and immunofluorescence analyses. PRG4 and MMP-13 expression were assessed to evaluate fibrocartilage integrity and degradation. Statistical significance was determined using ANOVA and post hoc testing.

Results: Female mice showed significantly higher Osteoarthritis Research Society International (OARSI) scores and MMP-13/PRG4 ratios at later time points, indicating sustained degradation. PRG4 expression increased similarly in both sexes in all time points, but MMP-13 expression was significantly higher in females at 9 weeks, suggesting heightened cartilage catabolism.

Conclusion: This study highlights sex-based differences in TMJ OA progression, reinforcing the importance of targeted therapeutic strategies. The burn-synovectomy model effectively replicates TMJ OA pathology, making it a valuable tool for future research.

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烧伤-滑膜切除颞下颌骨关节炎小鼠模型中纤维软骨退化差异的性别鉴定。

背景：颞下颌关节骨关节炎是一种退行性疾病，在女性中发病率较高，但其性别特异性机制尚不清楚。本研究在烧伤滑膜切除术TMJ OA小鼠模型中研究了纤维软骨降解，假设雌性小鼠由于不同的炎症反应而表现出更大的软骨降解。方法:PRG4-CreERT2;Ai9只小鼠（12周龄的雄性和雌性）接受烧伤滑膜切除术损伤，并在1、3和9周时安乐死，进行组织学和免疫荧光分析。评估PRG4和MMP-13的表达以评估纤维软骨的完整性和降解情况。采用方差分析和事后检验确定统计学显著性。结果：在较晚的时间点，雌性小鼠的骨关节炎国际研究学会（OARSI）评分和MMP-13/PRG4比值显著升高，表明持续退化。在所有时间点，PRG4的表达在两性中都有相似的增加，但MMP-13的表达在9周时在女性中显著增加，表明软骨分解代谢增加。结论：本研究强调了TMJ OA进展的性别差异，强调了靶向治疗策略的重要性。烧伤-滑膜切除模型有效地复制了TMJ骨性关节炎病理，为今后的研究提供了有价值的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Orthodontics & Craniofacial Research 医学-牙科与口腔外科

CiteScore

5.30

自引率

3.20%

发文量

审稿时长

>12 weeks

期刊介绍： Orthodontics & Craniofacial Research - Genes, Growth and Development is published to serve its readers as an international forum for the presentation and critical discussion of issues pertinent to the advancement of the specialty of orthodontics and the evidence-based knowledge of craniofacial growth and development. This forum is based on scientifically supported information, but also includes minority and conflicting opinions. The objective of the journal is to facilitate effective communication between the research community and practicing clinicians. Original papers of high scientific quality that report the findings of clinical trials, clinical epidemiology, and novel therapeutic or diagnostic approaches are appropriate submissions. Similarly, we welcome papers in genetics, developmental biology, syndromology, surgery, speech and hearing, and other biomedical disciplines related to clinical orthodontics and normal and abnormal craniofacial growth and development. In addition to original and basic research, the journal publishes concise reviews, case reports of substantial value, invited essays, letters, and announcements. The journal is published quarterly. The review of submitted papers will be coordinated by the editor and members of the editorial board. It is policy to review manuscripts within 3 to 4 weeks of receipt and to publish within 3 to 6 months of acceptance.