Stability of a blaGES-5-carrying plasmid in a Serratia marcescens nosocomial outbreak

IF 1.9 4区医学 Q3 INFECTIOUS DISEASES

Journal of Infection and Chemotherapy Pub Date : 2025-05-29 DOI:10.1016/j.jiac.2025.102742

Shoko Komatsu, Ryohei Nomoto, Noriko Nakanishi

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引用次数: 0

Abstract

In 2020, an outbreak of bla_GES-5-carrying plasmid, p2020-O-9, harboring S. marcescens occurred in an intensive care unit in Kobe City, Japan [1]. During the outbreak, strains with and without p2020-O-9 were simultaneously isolated from three patients; hence, we evaluate the stability of p2020-O-9 and its contribution to carbapenem resistance. Growth and transcriptional expression of bla_GES-5 were affected by the presence of p2020-O-9 at a meropenem concentration of at least 4 μg/mL. The bla_GES-5-harboring plasmid could be transformed into Escherichia coli via electroporation, but not conjugation. The p2020-O-9 plasmid was highly unstable, and complete loss was observed at the 25th serial passage when antibiotic pressure was withdrawn. Therefore, the bla_GES-5-harboring plasmid p2020-O-9 was unstable and non-conjugative, and contributed to meropenem resistance in a concentration-dependent manner in the previously reported S. marcescens nosocomial outbreak.

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粘质沙雷氏菌院内暴发中携带blages -5质粒的稳定性

2020年，在日本神户市的一个重症监护病房发生了携带blages -5质粒p2020-O-9携带粘质链球菌的疫情。疫情期间，从3名患者身上同时分离出携带和不携带p2020-O-9的菌株；因此，我们评估了p2020-O-9的稳定性及其对碳青霉烯抗性的贡献。p2020-O-9在美罗培南浓度≥4 μg/mL时影响blaGES-5的生长和转录表达。携带blages -5的质粒可以通过电穿孔转化到大肠杆菌中，但不能通过缀合转化。p2020-O-9质粒高度不稳定，在停用抗生素压力时，第25代质粒完全丢失。因此，携带blages -5的质粒p2020-O-9不稳定且非偶联性，在先前报道的黏质葡萄球菌医院暴发中以浓度依赖的方式促成了美罗培南耐药性。

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来源期刊

Journal of Infection and Chemotherapy INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

4.10

自引率

4.50%

发文量

303

审稿时长

47 days

期刊介绍： The Journal of Infection and Chemotherapy (JIC) — official journal of the Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases — welcomes original papers, laboratory or clinical, as well as case reports, notes, committee reports, surveillance and guidelines from all parts of the world on all aspects of chemotherapy, covering the pathogenesis, diagnosis, treatment, and control of infection, including treatment with anticancer drugs. Experimental studies on animal models and pharmacokinetics, and reports on epidemiology and clinical trials are particularly welcome.