Silencing PsASH2 affects embryo development in the cotton mealybug.

IF 2.3 2区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Molecular Biology Pub Date : 2025-05-30 DOI:10.1111/imb.13003

Kaixin Wang, Lingqi Zen, Lilu Sheng, Feihuang Lu, Qianjin Lin, Yifan Li, Haojie Tong

引用次数: 0

Abstract

Mealybugs are highly aggressive pests that infest various plants and cause substantial economic losses. Histone lysine methyltransferases (KMT) are evolutionarily conserved and proposed to be essential in early embryo development in animals. However, few KMTs have been reported in mealybugs. Here, we identified a novel KMT gene, PsASH2, in the cotton mealybug, Phenacoccus solenopsis Tinsley. This gene was highly expressed in the ovary of female adults. Through RNA interference (RNAi) of PsASH2 by dsRNA microinjection, we found a reduction in the number of male embryos and total embryos in the ovaries of pregnant females. Continuous downregulation of PsASH2 in mated females until their death resulted in few changes in sex ratio but significant decreases in the number of both male and female offspring. Therefore, we believe that PsASH2 plays essential roles in embryo survival for both sexes of the cotton mealybug which may provide a potential target gene for the management of cotton mealybug by disrupting embryo development.

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沉默PsASH2会影响棉粉蚧的胚胎发育。

粉蚧是一种极具侵略性的害虫，它侵害各种植物，造成重大的经济损失。组蛋白赖氨酸甲基转移酶（KMT）在进化上是保守的，在动物早期胚胎发育中起重要作用。然而，在粉虫中很少有kmt的报道。本研究在扶桑绵粉蚧（Phenacoccus solenopsis Tinsley）中鉴定出一种新的KMT基因PsASH2。该基因在雌性成虫卵巢中高度表达。我们通过dsRNA显微注射对PsASH2进行RNA干扰（RNAi），发现妊娠雌性卵巢内雄性胚胎数量和胚胎总数减少。在交配的雌性中，PsASH2持续下调直到死亡，导致性比例变化不大，但雄性和雌性后代的数量都显著减少。因此，我们认为PsASH2在棉粉蚧两性的胚胎存活中起着至关重要的作用，并可能通过破坏胚胎发育为棉粉蚧的管理提供潜在的靶基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Insect Molecular Biology 生物-昆虫学

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).