Surface-Enhanced Raman Scattering and Cell Imaging Ability of Plasmonic Tags Based on Covalent Conjugates of Gold Nanorods With Aromatic Alkynes

IF 2.4 3区化学 Q2 SPECTROSCOPY

Journal of Raman Spectroscopy Pub Date : 2025-03-10 DOI:10.1002/jrs.6791

Andrei I. Demenshin, Evgenii V. Borisov, Viktor N. Sorokoumov, Elena V. Solovyeva

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Abstract

The inherent benefits of Raman spectroscopy make it a suitable tool for the analysis of complex biological objects. Detection and imaging of mammalian cells is one of its development areas. Plasmonic tags endowed with alkyne reporter intended for bioimaging by surface-enhanced Raman scattering in the silent region are presented. Gold nanorods with covalently conjugated 4-aminotolane were obtained using dithiobis (succinimidyl propionate) and characterized by UV–Vis absorbance, transmission electron microscopy, and dynamic light scattering. Verification of surface amide bonds formation was performed with attenuated total reflectance and X-ray photoelectron spectroscopy. Surface-enhanced Raman scattering spectra of the tags were registered in various media and compared with a feedback from the similar system obtained through non-specific adsorption of 4-aminotolane on gold nanorods. Cytotoxicity of Raman reporter itself and its conjugates with gold nanorods were verified on HeLa cell line. Spectral mapping and scanning electron microscopy revealed the localized intracellular accumulation of the tags. The challenge associated with reduced imaging area due to formation of the tags aggregates inside the cells is pointed out.

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基于金纳米棒与芳香炔共价偶联物的等离子体标签的表面增强拉曼散射和细胞成像能力

拉曼光谱固有的优点使其成为分析复杂生物物体的合适工具。哺乳动物细胞的检测与成像是其发展方向之一。具有炔报告的等离子体标签，用于无声区表面增强拉曼散射的生物成像。采用丙酸琥珀酰亚胺酯制备了共价偶联4-氨基甲苯的金纳米棒，并用紫外可见吸收光谱、透射电镜和动态光散射对其进行了表征。用衰减全反射和x射线光电子能谱法验证了表面酰胺键的形成。在不同介质中记录了标签的表面增强拉曼散射光谱，并与通过4-氨基甲苯在金纳米棒上非特异性吸附获得的类似系统的反馈进行了比较。在HeLa细胞株上验证了拉曼报告蛋白本身及其与金纳米棒结合物的细胞毒性。光谱图谱和扫描电镜显示了标签的局部细胞内积累。指出了由于在细胞内形成标签聚集体而减少成像面积的挑战。

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来源期刊

Journal of Raman Spectroscopy 物理-光谱学

CiteScore

5.40

自引率

8.00%

发文量

185

审稿时长

3.0 months

期刊介绍： The Journal of Raman Spectroscopy is an international journal dedicated to the publication of original research at the cutting edge of all areas of science and technology related to Raman spectroscopy. The journal seeks to be the central forum for documenting the evolution of the broadly-defined field of Raman spectroscopy that includes an increasing number of rapidly developing techniques and an ever-widening array of interdisciplinary applications. Such topics include time-resolved, coherent and non-linear Raman spectroscopies, nanostructure-based surface-enhanced and tip-enhanced Raman spectroscopies of molecules, resonance Raman to investigate the structure-function relationships and dynamics of biological molecules, linear and nonlinear Raman imaging and microscopy, biomedical applications of Raman, theoretical formalism and advances in quantum computational methodology of all forms of Raman scattering, Raman spectroscopy in archaeology and art, advances in remote Raman sensing and industrial applications, and Raman optical activity of all classes of chiral molecules.