Development of pectin-gelatin biopolymer films with DES based Chlorella vulgaris extracts as plasticizers for enhanced UV-blocking, antioxidant, and antimicrobial food packaging application

IF 10.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Packaging and Shelf Life Pub Date : 2025-06-01 DOI:10.1016/j.fpsl.2025.101534

Gulsah Karabulut , Gulden Goksen

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引用次数: 0

Abstract

The growing demand for alternatives to synthetic plastic in food packaging has driven the development of multifunctional biopolymer films. This study explores the integration of Deep Eutectic Solvents (DES) and bioactive compounds from Chlorella vulgaris into pectin-gelatin films to enhance their mechanical, bioactive, and barrier properties for active food packaging applications. Films were prepared using glycerol, DES, or DES enriched with C. vulgaris (ADES) as plasticizers at 20 % and 40 % concentrations. ADES films exhibited the most significant enhancements, with tensile strength increasing by 35 % and elongation at break by 22 % compared to DES films. FTIR and XRD data revealed enhanced hydrogen bonding and crystallinity due to interactions between C. vulgaris bioactives and the polymer matrix. Optical analysis revealed that glycerol-based films exhibited the highest transparency but provided minimal UV-blocking. In contrast, DES-based films showed moderate opacity and UV protection, while ADES-based films—enriched with C. vulgaris bioactives—displayed the highest opacity and the most effective UV-blocking capability. ADES films demonstrated superior antioxidant activity and total phenolic content. Antimicrobial assays showed broad-spectrum activity, with inhibition zones against S. aureus and E. coli increasing by 20 % and 80 % in 40AD films. Food application tests on chicken meat highlighted the efficacy of ADES films in maintaining pH stability and reducing lipid oxidation by 41 %. Moreover, microbial growth was significantly suppressed, with total mesophilic bacteria reduced to 5.59 log CFU/g after 7 days. These findings demonstrate the potential of ADES-based films as bioactive packaging materials, offering multifunctionality and improved shelf life for perishable foods.

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以DES为基础的普通小球藻提取物作为增塑剂的果胶-明胶生物聚合物薄膜的开发，用于增强食品包装的防紫外线、抗氧化和抗菌应用

食品包装中对合成塑料替代品的需求日益增长，推动了多功能生物聚合物薄膜的发展。本研究探讨了将普通小球藻的深共晶溶剂（DES）和生物活性化合物整合到果胶-明胶薄膜中，以提高其机械、生物活性和阻隔性能，用于活性食品包装。分别以甘油、DES或富含丙烯醚（ADES）的DES作为增塑剂，分别以20 %和40 %的浓度制备薄膜。与DES薄膜相比，ADES薄膜的抗拉强度提高了35 %，断裂伸长率提高了22 %。红外光谱（FTIR）和x射线衍射（XRD）数据显示，由于生物活性物质与聚合物基质的相互作用，导致了氢键和结晶度的增强。光学分析显示，甘油基薄膜具有最高的透明度，但提供最小的紫外线阻挡。相反，基于des的膜具有中等的不透明度和紫外线防护能力，而基于ads的膜具有最高的不透明度和最有效的紫外线防护能力，因为ads富含寻常草的生物活性。ADES薄膜表现出优异的抗氧化活性和总酚含量。抗菌试验显示广谱活性，40AD膜对金黄色葡萄球菌和大肠杆菌的抑制区分别增加了20% %和80% %。对鸡肉的食品应用试验表明，ADES薄膜在保持pH稳定和减少脂质氧化方面的效果为41% %。微生物生长明显受到抑制，7天后中温细菌总数降至5.59 log CFU/g。这些发现证明了基于ades的薄膜作为生物活性包装材料的潜力，为易腐食品提供了多功能和延长的保质期。

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来源期刊

Food Packaging and Shelf Life Agricultural and Biological Sciences-Food Science

CiteScore

14.00

自引率

8.80%

发文量

214

审稿时长

70 days

期刊介绍： Food packaging is crucial for preserving food integrity throughout the distribution chain. It safeguards against contamination by physical, chemical, and biological agents, ensuring the safety and quality of processed foods. The evolution of novel food packaging, including modified atmosphere and active packaging, has extended shelf life, enhancing convenience for consumers. Shelf life, the duration a perishable item remains suitable for sale, use, or consumption, is intricately linked with food packaging, emphasizing its role in maintaining product quality and safety.