Plasmonic Optical Fibre–Based Point-of-Care Test for Periodontal MIP-1α Detection: A Validation Study of a Multiplexed Biosensor Prototype

IF 3.2 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International dental journal Pub Date : 2025-06-02 DOI:10.1016/j.identj.2025.04.010

Marco Annunziata , Francesco Arcadio , Emanuela Stampone , Debora Bencivenga , Gennaro Cecoro , Chiara Marzano , Angelantonio Piccirillo , Fulvio Della Ragione , Nunzio Cennamo , Luigi Zeni , Adriana Borriello , Luigi Guida

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引用次数: 0

Abstract

Introduction

The aim of this study was to assess the analytical performance of a multiplexed plasmonic optical fibre–based point-of-care test (POCT) in detecting and quantifying salivary macrophage inflammatory protein-1 alpha (MIP-1α) by comparing it with a standard enzyme-linked immunosorbent assay (ELISA).

Methods

Three plastic optical fibres (POFs) were modified to host a self-assembled monolayer (SAM) of anti-MIP-1α antibodies. Each of them was interposed between a light source and a spectrometer to detect the variations in the refractive index at the POF-SAM interface caused by surface plasmon resonance (SPR) when the antibody-analyte binding occurred. A dose-response Langmuir fitting curve, with MIP-1α dilutions from 0.25 to 10 pM, was calculated. Fifty salivary samples from consecutively enrolled subjects were tested by the SPR-POF biosensor and ELISA, and the obtained values were compared (Spearman’s rank correlation test). Differences in MIP-1α levels among patients based on age, gender and the presence or absence of periodontitis were also analysed (Mann-Whitney U test).

Results

A strong positive correlation between SPR-POF and ELISA measurements was found (Spearman’s r_s = 0.894, P < .001). The SPR-POF limit of detection (LoD) was 0.15 pM, even lower than the ELISA LoD (0.78 pM). Higher (P < .05) MIP-1α levels in periodontitis compared to non-periodontitis patients were found.

Conclusions

The developed 3-arm plasmonic POCT performed comparably to ELISA in detecting and quantifying salivary MIP-1α, both in terms of LoD and accuracy, with measurement rapidity and reliability enhanced by the multiplexed 3-arm design.

Clinical Relevance

The novel SPR-POF prototype would be of value for future studies related to POCT devices for monitoring of periodontal health and disease.

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基于等离子体光纤的牙周MIP-1α检测：多路生物传感器原型的验证研究

本研究的目的是通过与标准酶联免疫吸附试验（ELISA）比较，评估基于多路等离子体光纤的即时检测（POCT）在检测和定量唾液巨噬细胞炎症蛋白-1α (MIP-1α)方面的分析性能。方法对三种塑料光纤（POFs）进行修饰，使其承载抗mip -1α抗体的自组装单层（SAM）。将它们分别置于光源和光谱仪之间，以检测抗体与分析物结合时，表面等离子体共振（SPR）引起的POF-SAM界面折射率的变化。计算MIP-1α浓度为0.25 ~ 10 pM时的剂量-响应Langmuir拟合曲线。采用SPR-POF生物传感器和酶联免疫吸附试验（ELISA）检测50份连续入组受试者的唾液样本，并比较所得值（Spearman秩相关检验）。MIP-1α水平在年龄、性别和有无牙周炎患者中的差异也被分析（Mann-Whitney U检验）。结果SPR-POF与ELISA检测结果呈显著正相关(Spearman’s rs = 0.894, P <；措施)。SPR-POF的检出限（LoD）为0.15 pM，甚至低于ELISA的LoD （0.78 pM）。更高(P <；.05)牙周炎患者与非牙周炎患者的MIP-1α水平比较。结论建立的三臂等离子体POCT检测和定量唾液MIP-1α的方法在LoD和准确性上与ELISA相当，多臂设计提高了检测的速度和可靠性。新型的SPR-POF原型将对未来用于监测牙周健康和疾病的POCT设备的研究有价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International dental journal 医学-牙科与口腔外科

CiteScore

4.80

自引率

6.10%

发文量

159

审稿时长

63 days

期刊介绍： The International Dental Journal features peer-reviewed, scientific articles relevant to international oral health issues, as well as practical, informative articles aimed at clinicians.