Sexually Dimorphic Cellular Architecture and Neural Circuity of ovBNST Proenkephalin Neurons.

IF 5.9 2区 医学 Q1 NEUROSCIENCES
Limei Song, Yuqing Zhang, Mengqi Feng, Wenwen Su, Riming Zhu, Bin Zhang, Xia Zhang, Jie Li
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Abstract

Sexual dimorphism in the brain underlies behavioral differences between sexes. The bed nucleus of the stria terminalis (BNST) is a complex nucleus that differs between males and females, but the sexual dimorphism in cytoarchitecture and the connectome of its oval subdivision (ovBNST) remains largely unexplored. By combining snRNA-seq and transgenic labeling, we found a higher density of ovBNST proenkephalin (ovBNSTPENK) neurons in male than female mice. Anatomically, we virally mapped the efferents and afferents of ovBNSTPENK neurons, finding reciprocally dimorphic connections with the hypothalamus and striatum. Gene enrichment analysis suggests that ovBNSTPENK neurons are modulated by the upstream dopamine pathway. Functionally, by applying caspase-3-mediated depletion of ovBNSTPENK neurons, we found that loss of these neurons enhanced locomotor activity in male but not female mice, without altering the anxiety-like phenotypes in either sex. Our study may pave the way for a better understanding of the anatomical and functional profiles of ovBNSTPENK neurons from a sexually dimorphic perspective.

ovBNST前脑啡肽神经元的两性二态细胞结构和神经回路。
大脑中的两性二态性是两性行为差异的基础。终纹床核(BNST)是一个复杂的核,在男性和女性之间存在差异,但细胞结构的性别二态性及其卵形分支的连接组(ovBNST)仍未得到充分研究。结合snRNA-seq和转基因标记,我们发现雄性小鼠的ovBNSTPENK神经元密度高于雌性小鼠。在解剖学上,我们绘制了ovBNSTPENK神经元的传出和传入信号图,发现了与下丘脑和纹状体的相互二态连接。基因富集分析表明ovBNSTPENK神经元受上游多巴胺通路调节。在功能上,通过应用caspase-3介导的ovBNSTPENK神经元的缺失,我们发现这些神经元的缺失在雄性小鼠中增强了运动活动,而在雌性小鼠中没有,没有改变雌雄小鼠的焦虑样表型。我们的研究可能为从两性二态的角度更好地理解ovBNSTPENK神经元的解剖和功能特征铺平道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Neuroscience bulletin
Neuroscience bulletin NEUROSCIENCES-
CiteScore
7.20
自引率
16.10%
发文量
163
审稿时长
6-12 weeks
期刊介绍: Neuroscience Bulletin (NB), the official journal of the Chinese Neuroscience Society, is published monthly by Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS) and Springer. NB aims to publish research advances in the field of neuroscience and promote exchange of scientific ideas within the community. The journal publishes original papers on various topics in neuroscience and focuses on potential disease implications on the nervous system. NB welcomes research contributions on molecular, cellular, or developmental neuroscience using multidisciplinary approaches and functional strategies. We feature full-length original articles, reviews, methods, letters to the editor, insights, and research highlights. As the official journal of the Chinese Neuroscience Society, which currently has more than 12,000 members in China, NB is devoted to facilitating communications between Chinese neuroscientists and their international colleagues. The journal is recognized as the most influential publication in neuroscience research in China.
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