Comparative study of mcr-10 plasmids in Enterobacter spp. with the mcr-10_ter locus from wastewater and clinical samples: Implications for antimicrobial resistance and fitness

IF 3.2 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2025-05-28 DOI:10.1016/j.jgar.2025.05.021

Liansheng Yu , Shizuo Kayama , Wataru Hayashi, Yo Sugawara, Sayoko Kawakami, Motoyuki Sugai

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引用次数: 0

Abstract

Objectives

The emergence and dissemination of mobile colistin resistance (mcr) genes among various Enterobacter species found in humans, animals, and the environment have attracted significant global attention. We report the emergence of mcr-10-carrying plasmids in Enterobacter spp. isolated from wastewater in Japan. This study aimed to characterize the genetic environment of mcr-10 detected in Japan.

Methods

Wastewater samples were collected bimonthly from four municipal wastewater treatment plants in Hiroshima, Japan between October 2020 and August 2021, and three mcr-10-harboring isolates were recovered. These isolates were characterized by antimicrobial susceptibility testing, whole-genome sequencing, and comparative analysis of mcr-10-carrying plasmids.

Results

The three colistin-resistant strains, JARB-T-NT00029, JARB-T-NT00194, and JARB-T-NT00318 belonged to sequence types 364, 32, and 486, respectively. The mcr-10 genes were located on 118–155 kb plasmids of the IncFIB(K), IncFIA(HI1), and IncFIB(pECLA)/IncFII(pECLA) replicon types. These plasmids showed high similarity to mcr-10-carrying plasmids found in Enterobacter spp. isolated from human samples reported in other countries across Asia and Europe. Further detailed comparative analysis revealed that an mcr-10_ter locus–containing fragment, including genes involved in colistin resistance and the promotion of gut colonization, was conserved in 14 of 30 (46.7%) strains isolated from human samples in Japan.

Conclusions

We reported colistin-resistant Enterobacter spp. harboring plasmids carrying the mcr-10. This is the first detailed characterization of the genetic structures of the mcr-10-carrying plasmids isolated from the environment in Japan. Our investigation suggests the potential spread of a combination of genes for colistin resistance and the promotion of gut colonization.

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肠杆菌中mcr-10质粒与废水和临床样品中mcr-10_ter位点的比较研究：对抗菌素耐药性和适应性的影响

目的：在人类、动物和环境中发现的各种肠杆菌中出现和传播移动粘菌素耐药性（mcr）基因引起了全球的广泛关注。我们报道从日本废水中分离的肠杆菌中出现携带mcr-10的质粒。本研究旨在对在日本检测到的mcr-10的遗传环境进行表征。方法：于2020年10月至2021年8月，对日本广岛市4个城市污水处理厂的废水取样，每两个月采集一次，回收3株携带mcr-10的分离株。通过抗菌药敏试验、全基因组测序和携带mcr-10质粒的比较分析对这些分离株进行了鉴定。结果：3株耐粘菌素菌株JARB-T-NT00029、JARB-T-NT00194和JARB-T-NT00318分别属于序列型364、32和486。mcr-10基因位于IncFIB(K)、IncFIA（HI1）和IncFIB(pECLA)/IncFII（pECLA）复制子类型的118 ~ 155 kb质粒上。这些质粒与从亚洲和欧洲其他国家报告的人类样本分离的肠杆菌中发现的携带mc10的质粒高度相似。进一步详细的比较分析显示，在日本从人类样本分离的30株菌株中，有14株（46.7%）保存了含有mcr-10_ter基因座的片段，其中包括参与粘菌素耐药性和促进肠道定植的基因。结论：我们报道了耐粘菌素肠杆菌携带携带mcr-10的质粒。这是首次详细描述从日本环境中分离的携带mcr-10的质粒的遗传结构。我们的研究表明，粘菌素耐药基因组合的潜在传播和促进肠道定植。

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.