T P Makalish, N N Pilyavskiy, I O Golovkin, G V Maslova
{"title":"Proportion of Immunoregulatory Cells in Inflammatory Infiltrate in Rheumatoid Arthritis Model and Its Correction by Antisense Oligonucleotides.","authors":"T P Makalish, N N Pilyavskiy, I O Golovkin, G V Maslova","doi":"10.1007/s10517-025-06407-w","DOIUrl":null,"url":null,"abstract":"<p><p>Autoimmune inflammation in rheumatoid arthritis is caused by various immunocompetent cells secreting a wide range of cytokines. Macrophages, CD4<sup>+</sup>, and CD8<sup>+</sup> T cells are the most active in this process. We propose using antisense oligonucleotides (ASO) that block the synthesis of TNFα and IL-6. In the adjuvant-induced arthritis model, changes in the number of CD4<sup>+</sup>, CD8<sup>+</sup>, and CD68<sup>+</sup> cells in the inflammatory infiltrate after correction with ASO were assessed. The number of immunopositive inflammatory infiltrate cells in the area of bone damage was counted. CD8<sup>+</sup> cells turned out to be the most numerous. Blockade of IL-6 led to a statistically significant decrease in the number of CD4<sup>+</sup> cells. The use of ASO reduced the number of macrophages. The combination of ASO against IL-6 and TNFα administered by electrophoresis most effective.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"733-738"},"PeriodicalIF":0.9000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-025-06407-w","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/30 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
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Abstract
Autoimmune inflammation in rheumatoid arthritis is caused by various immunocompetent cells secreting a wide range of cytokines. Macrophages, CD4+, and CD8+ T cells are the most active in this process. We propose using antisense oligonucleotides (ASO) that block the synthesis of TNFα and IL-6. In the adjuvant-induced arthritis model, changes in the number of CD4+, CD8+, and CD68+ cells in the inflammatory infiltrate after correction with ASO were assessed. The number of immunopositive inflammatory infiltrate cells in the area of bone damage was counted. CD8+ cells turned out to be the most numerous. Blockade of IL-6 led to a statistically significant decrease in the number of CD4+ cells. The use of ASO reduced the number of macrophages. The combination of ASO against IL-6 and TNFα administered by electrophoresis most effective.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
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