Clonogenic Capacity and Migration Potential of A549 and HeLa Cell Lines Survived after Acute X-Ray Irradiation at a Dose of 15 Gy.

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-04-01 Epub Date: 2025-05-30 DOI:10.1007/s10517-025-06413-y

A A Tsishnatti, S M Rodneva, Yu A Fedotov, V D Gurev, V N Belyaev, D V Guryev

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Abstract

We obtained A549 and HeLa cell lines that survived and showed stable growth after acute X-ray irradiation at a dose of 15 Gy (hereinafter referred to as A549RR and HeLaRR). Comparative studies of the proliferative activity of original (A549, HeLa) and survived (A549RR, HeLaRR) cells and their sensitivity to additional X-ray irradiation at doses of 2, 4, and 6 Gy by the clonogenic capacity and migration potential (scratch-wound test) were carried out. It was found that the doubling time of the cell population for the A549RR and HeLaRR lines was significantly lower than for the original non-irradiated cells without additional exposure. A549RR and HeLaRR cell lines demonstrated higher resistance to additional X-ray irradiation in terms of clonogenic ability. Migration potential, as one of the critical processes during tumor metastasis, appeared to be significantly higher for A549RR cells up to 24 h after irradiation at a dose of 2 Gy and up to 48 h after irradiation at a dose of 4 Gy. Migration of HeLaRR cells was significantly higher without additional exposure and after irradiation at doses of 2 and 4 Gy, while after irradiation at a dose of 6 Gy, migration activity of HeLaRR cells was significantly suppressed compared to original cells.

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15gy急性x射线照射后A549和HeLa细胞系的克隆生成能力和迁移潜力

我们获得了A549和HeLa细胞系，经过15 Gy剂量的急性x射线照射后存活并稳定生长（以下简称A549RR和HeLaRR）。对原始（A549, HeLa）和存活（A549RR, HeLaRR）细胞的增殖活性及其对2、4和6 Gy剂量x射线额外照射的敏感性进行了克隆生成能力和迁移潜力（划伤试验）的比较研究。结果发现，A549RR和HeLaRR细胞系的细胞群倍增时间明显低于未照射的原始细胞。在克隆生成能力方面，A549RR和HeLaRR细胞系对额外x射线照射表现出更高的抗性。作为肿瘤转移过程中的关键过程之一，A549RR细胞在2 Gy照射后24 h和4 Gy照射后48 h的迁移电位明显升高。2 Gy和4 Gy辐照后，HeLaRR细胞的迁移能力明显增强，而6 Gy辐照后，HeLaRR细胞的迁移能力明显受到抑制。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.