Protective and regenerative roles of novel probiotic strains in mitigating cyclophosphamide-induced genotoxicity and organ damage in mice

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-05-28 DOI:10.1016/j.genrep.2025.102271

Entesar E. Hassan , Noha E. Ibrahim , Medhat M. Menshawy , Ahmed M. Darwish , Abd-El Nasser A. Khattab , Ayman A. Farghaly

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Abstract

This study evaluated the protective effects of two probiotic strains, Lactobacillus fermentum (Pro5) and Lactobacillus casei (Pro3), isolated from silage and Balady butter, against cyclophosphamide (CP)-induced genotoxicity and organ damage a study was conducted using 80 male albino mice of the Swiss strain were categorized into eight groups and administered oral treatments over a period of seven days, including the control group, Pro5 group, pro3 group at a dose of 2 × 10⁹ CFU/mL, Pro5 + Pro3(mix) group at a dose of 2 × 10⁹ CFU/mL, cyclophosphamide (CP) group (20 mg/kg b.w), Pro5+ CP, Pro3+ CP, Mix+CP. Blood and tissue samples were analyzed for chromosomal aberrations, histopathological changes, and gene expression of TNFα, TNFS, and IL-22. Results showed that Pro5 and Pro3 significantly reduced CP-induced chromosomal aberrations in bone marrow and spleen cells, with effects comparable to the control group. Histopathological analysis revealed severe liver damage in the CP group, including hepatocyte degeneration and inflammation, while Pro5, Pro3, and Mix groups exhibited near-normal liver structure with mild inflammation. Similar improvements were observed in kidney and spleen tissues, with probiotics mitigating CP-induced damage. Gene expression analysis indicated that TNFα and TNFs were significantly upregulated in the CP group but downregulated in Pro3, Mix, and Mix+CP groups. IL-22 expression increased significantly in probiotic-treated groups, suggesting enhanced immune regulation. These findings demonstrate the protective and regenerative potential of Pro5 and Pro3 against CP-induced genotoxicity and organ damage, highlighting their ability to modulate inflammatory and immune responses effectively.

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新型益生菌菌株在减轻环磷酰胺诱导的小鼠遗传毒性和器官损伤中的保护和再生作用

从青贮饲料和Balady黄油中分离的发酵乳杆菌（Pro5）和干酪乳杆菌（Pro3）两种益生菌对环磷酰胺（CP）引起的遗传毒性和器官损伤的保护作用进行了研究。将80只雄性白化小鼠瑞士菌株分为8组，分别给予对照组、Pro5组、Pro3组，剂量为2 × 109 CFU/mL。Pro5+ Pro3（mix）组剂量为2 × 109 CFU/mL，环磷酰胺（CP）组剂量为20 mg/kg b.w, Pro5+ CP、Pro3+ CP、mix +CP。分析血液和组织样本的染色体畸变、组织病理变化以及TNFα、TNFS和IL-22的基因表达。结果显示，Pro5和Pro3显著降低了cp诱导的骨髓和脾脏细胞的染色体畸变，效果与对照组相当。组织病理学分析显示，CP组肝脏损伤严重，包括肝细胞变性和炎症，而Pro5、Pro3和Mix组肝脏结构接近正常，伴有轻度炎症。在肾脏和脾脏组织中观察到类似的改善，益生菌减轻了cp引起的损伤。基因表达分析显示，CP组TNFα和tnf显著上调，而Pro3、Mix和Mix+CP组则下调。益生菌处理组IL-22表达显著升高，提示免疫调节增强。这些发现证明了Pro5和Pro3对cp诱导的遗传毒性和器官损伤的保护和再生潜力，突出了它们有效调节炎症和免疫反应的能力。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.