A network of RNA-binding and metabolic proteins evidenced in the Entamoeba histolytica nuclear proteome

Abstract

Amoebiasis caused by the protozoan parasite Entamoeba histolytica is a serious leading cause of parasite-related death worldwide and there is a need for developing new safe and efficient control methods. The nuclear polyadenylation factor EhCFIm25 has been recently reported as a biochemical target in E. histolytica, being necessary for proliferation and parasite virulence. Interestingly, the prediction and experimental characterization of EhCFIm25 interactome revealed close relationships between nuclear proteins involved in gene expression regulation and typically cytoplasmic enzymes of redox and energy metabolism. Here, the description of the nuclear proteome of E. histolytica obtained by mass spectrometry analysis confirmed the presence of metabolic proteins in the nuclear compartment, which could have moonlight functions. Data are available via ProteomeXchange with identifier PXD056950. The predicted network of RNA-binding and metabolic proteins also evidenced the relevance of five metabolic enzymes in the coordination between energy and RNA metabolism, opening new perspectives for amoebiasis control.