Methyl jasmonate activates an OPR gene that regulates the β-citronellol synthesis in herbaceous peony

IF 5.6 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-05-31 DOI:10.1016/j.indcrop.2025.121294

Qian Zhao , Yuqing Li , Lina Gu , Di He , Chenxi Wei , Jianrang Luo , Yanlong Zhang

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引用次数: 0

Abstract

Floral scent is a key ornamental trait in plants, and exogenous hormonal treatments can enhance its intensity. In this study, we demonstrated that exogenous methyl jasmonate (MeJA) enhanced floral fragrance intensity in herbaceous peony (Paeonia lactiflora), mainly by elevating β-citronellol content. Application of MeJA at the S2 (half-opened) stage resulted in the highest fragrance intensity during the full-blooming phase. The 12-oxophytodienoate reductase (OPR) genes are involved in β-citronellol synthesis. We identified the OPR family in herbaceous peony and found five OPR genes, all of which were induced by MeJA. Functional analysis revealed that PlOPR2, PlOPR3, and PlOPR5 promote β-citronellol synthesis, among which PlOPR2 is the most potent, while PlOPR1 drives endogenous jasmonic acid biosynthesis. MeJA promoted β-citronellol synthesis via the CGTCA motif within the PlOPR2 promoter. This study provides novel insights into the regulation of β-citronellol in herbaceous peony.

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茉莉酸甲酯激活调控牡丹β-香茅醇合成的OPR基因

花香是植物重要的观赏性状，外源激素处理可以增强花香的强度。在本研究中，我们证明外源茉莉酸甲酯（MeJA）主要通过提高β-香茅醇含量来增强芍药（Paeonia lactiflora）花香强度。在S2（半开期）施用MeJA，在开花期香气强度最高。12-氧植物二烯酸还原酶（OPR）基因参与了β-香茅醇的合成。对芍药OPR家族进行鉴定，发现5个OPR基因，均为MeJA诱导的基因。功能分析显示，PlOPR2、PlOPR3和PlOPR5促进β-香茅醇合成，其中PlOPR2作用最强，PlOPR1促进内源性茉莉酸生物合成。MeJA通过PlOPR2启动子内的CGTCA基序促进β-香茅醇合成。本研究为揭示β-香茅醇在芍药中的调控作用提供了新的思路。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.