Dual-Responsive Fluorescent Sensor for Monitoring ALP and SO2 in Cholestatic Liver Injury Evaluation and Drug Screening

IF 8.3 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Applied Materials & Interfaces Pub Date : 2025-05-31 DOI:10.1021/acsami.5c06961

Yudi Cheng, Jiao Chen, Wenwen Jiang, Wenqi Li, Zhaohui Wang, Fulin Chen, Xiuying Xie, Jianli Li

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引用次数: 0

Abstract

Cholestatic liver injury (CLI) is a pathological condition characterized by impaired bile flow and elevated levels of oxidative stress. Its clinical manifestations are often asymptomatic in the early stage, while prolonged CLI may progress to liver failure without timely intervention. However, current diagnostic and therapeutic approaches for CLI are limited in both effectiveness and availability, highlighting the critical need for reliable diagnostic modalities and innovative therapeutic agents. Given that the alkaline phosphatase (ALP) and sulfur dioxide (SO₂) serve as pivotal indicators in CLI pathogenesis, here we constructed a dual-responsive fluorescent sensor N–P for detecting ALP and SO₂ with dual-channel signals. This sensor has excellent water solubility, a low limit of detection (LOD = 0.5458 U/L for ALP and 0.1604 μM for SO₂), and a large Stokes shift (∼170 nm). Moreover, N–P enables real-time visualization of endogenous ALP and SO₂ in living cells and zebrafish. Significantly, N–P serves as a robust platform for the visual screening of potential CLI therapeutic drugs and ALP/SO₂ activators/inhibitors, as well as for the early diagnosis of CLI. This work presents a versatile fluorescent sensor for the sequential detection of ALP and SO₂, thereby contributing to the advancement in pharmaceutical development and diagnostic approaches for CLI.

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双响应荧光传感器监测ALP和SO2在胆汁淤积性肝损伤评价和药物筛选中的应用

胆汁淤积性肝损伤（CLI）是一种以胆汁流动受损和氧化应激水平升高为特征的病理状态。其临床表现在早期往往无症状，而长期的CLI如不及时干预，可能发展为肝功能衰竭。然而，目前的诊断和治疗方法在有效性和可用性方面都是有限的，这突出了对可靠的诊断方式和创新治疗剂的迫切需要。鉴于碱性磷酸酶（ALP）和二氧化硫（SO2）是CLI发病机制中的关键指标，我们构建了双响应型荧光传感器N-P，通过双通道信号检测ALP和SO2。该传感器具有良好的水溶性，低检测限（ALP的LOD = 0.5458 U/L， SO2的LOD = 0.1604 μM）和大Stokes位移（~ 170 nm）。此外，N-P可以实时可视化活细胞和斑马鱼中的内源性ALP和SO2。值得注意的是，N-P可作为一个强大的平台，用于视觉筛选潜在的CLI治疗药物和ALP/SO2激活剂/抑制剂，以及CLI的早期诊断。这项工作提出了一种用于ALP和SO2序列检测的多功能荧光传感器，从而促进了CLI药物开发和诊断方法的进步。

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来源期刊

ACS Applied Materials & Interfaces 工程技术-材料科学：综合

CiteScore

16.00

自引率

6.30%

发文量

4978

审稿时长

1.8 months

期刊介绍： ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.