Positive Correlation between Lipin-1 and Lipin-2 Expressions and Hepatic T1 Values in IUGR Rats.

IF 1.1 4区 医学 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Tao Wang, MingZhu Deng, Alpha Kalonda Mutamba, XiaoRi He, Jing Bian, DuJun Bian
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引用次数: 0

Abstract

Background: Intrauterine growth restriction (IUGR) is associated with long-term metabolic disturbances, including obesity. Changes in hepatic lipid metabolism and adipose tissue function, mediated by lipin-1 and lipin-2, may contribute to these outcomes.

Aim: This study aimed to investigate the correlation between lipin-1 in visceral adipose tissues (VATs) and lipin-2 in the liver. It also examined hepatic T1 values using T1 mapping in IUGR rats.

Objective: The objective of this study was to explore the metabolic mechanisms linking IUGR and adult obesity by analyzing molecular and imaging markers.

Methods: Pregnant rats were fed either a low-protein diet (10%) to induce IUGR or a normal-protein diet (21%) as a control. Male offspring underwent conventional magnetic resonance imaging and native T1 mapping using a 3.0 T whole-body MR scanner at days 21, 56, and 84 post-birth. Liver tissues and VATs were collected for analysis. Lipin-1 and lipin-2 expression levels were measured using Western blot and real-time quantitative PCR.

Results: The IUGR group exhibited significantly higher mRNA and protein expression levels of lipin-1 and lipin-2 compared to the control group at days 21, 56, and 84 after birth. Additionally, the IUGR group demonstrated significantly higher hepatic T1 values than the control group at the corresponding time points. Positive correlations were observed between the protein and mRNA expression levels of lipin-1 and hepatic T1 values. Similarly, the protein and mRNA expression levels of lipin-2 were positively correlated with hepatic T1 values. All results were statistically significant (P<0.05).

Conclusion: The upregulation of lipin-1 and lipin-2 expressions was found to be linked to elevated hepatic T1 values, potentially contributing to adult obesity in IUGR rats.

IUGR大鼠肝脏T1值与Lipin-1、Lipin-2表达呈正相关。
背景:宫内生长受限(IUGR)与包括肥胖在内的长期代谢紊乱有关。由脂质-1和脂质-2介导的肝脏脂质代谢和脂肪组织功能的变化可能有助于这些结果。目的:探讨肝脏脂素-2与内脏脂肪组织(VATs)脂素-1的相关性。采用T1作图法检测IUGR大鼠肝脏T1值。目的:通过分析IUGR与成人肥胖的分子和影像学标志物,探讨IUGR与成人肥胖的代谢机制。方法:采用低蛋白饮食(10%)和正常蛋白饮食(21%)作为对照,诱导妊娠大鼠IUGR。雄性子代在出生后第21天、第56天和第84天使用3.0 T全身磁共振扫描仪进行常规磁共振成像和原生T1定位。收集肝组织和vat进行分析。采用Western blot和实时定量PCR检测Lipin-1和lipin-2的表达水平。结果:IUGR组在出生后第21天、第56天和第84天的脂素-1和脂素-2 mRNA和蛋白表达水平明显高于对照组。此外,IUGR组在相应时间点的肝脏T1值明显高于对照组。脂素-1蛋白和mRNA表达水平与肝脏T1值呈正相关。同样,脂素-2蛋白和mRNA表达水平与肝脏T1值呈正相关。结论:脂质-1和脂质-2表达上调与肝脏T1值升高有关,可能导致IUGR大鼠成年肥胖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.60
自引率
0.00%
发文量
246
审稿时长
1 months
期刊介绍: Current Medical Imaging Reviews publishes frontier review articles, original research articles, drug clinical trial studies and guest edited thematic issues on all the latest advances on medical imaging dedicated to clinical research. All relevant areas are covered by the journal, including advances in the diagnosis, instrumentation and therapeutic applications related to all modern medical imaging techniques. The journal is essential reading for all clinicians and researchers involved in medical imaging and diagnosis.
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