{"title":"Positive Correlation between Lipin-1 and Lipin-2 Expressions and Hepatic T1 Values in IUGR Rats.","authors":"Tao Wang, MingZhu Deng, Alpha Kalonda Mutamba, XiaoRi He, Jing Bian, DuJun Bian","doi":"10.2174/0115734056296733250514081236","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Intrauterine growth restriction (IUGR) is associated with long-term metabolic disturbances, including obesity. Changes in hepatic lipid metabolism and adipose tissue function, mediated by lipin-1 and lipin-2, may contribute to these outcomes.</p><p><strong>Aim: </strong>This study aimed to investigate the correlation between lipin-1 in visceral adipose tissues (VATs) and lipin-2 in the liver. It also examined hepatic T1 values using T1 mapping in IUGR rats.</p><p><strong>Objective: </strong>The objective of this study was to explore the metabolic mechanisms linking IUGR and adult obesity by analyzing molecular and imaging markers.</p><p><strong>Methods: </strong>Pregnant rats were fed either a low-protein diet (10%) to induce IUGR or a normal-protein diet (21%) as a control. Male offspring underwent conventional magnetic resonance imaging and native T1 mapping using a 3.0 T whole-body MR scanner at days 21, 56, and 84 post-birth. Liver tissues and VATs were collected for analysis. Lipin-1 and lipin-2 expression levels were measured using Western blot and real-time quantitative PCR.</p><p><strong>Results: </strong>The IUGR group exhibited significantly higher mRNA and protein expression levels of lipin-1 and lipin-2 compared to the control group at days 21, 56, and 84 after birth. Additionally, the IUGR group demonstrated significantly higher hepatic T1 values than the control group at the corresponding time points. Positive correlations were observed between the protein and mRNA expression levels of lipin-1 and hepatic T1 values. Similarly, the protein and mRNA expression levels of lipin-2 were positively correlated with hepatic T1 values. All results were statistically significant (P<0.05).</p><p><strong>Conclusion: </strong>The upregulation of lipin-1 and lipin-2 expressions was found to be linked to elevated hepatic T1 values, potentially contributing to adult obesity in IUGR rats.</p>","PeriodicalId":54215,"journal":{"name":"Current Medical Imaging Reviews","volume":" ","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Medical Imaging Reviews","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2174/0115734056296733250514081236","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Intrauterine growth restriction (IUGR) is associated with long-term metabolic disturbances, including obesity. Changes in hepatic lipid metabolism and adipose tissue function, mediated by lipin-1 and lipin-2, may contribute to these outcomes.
Aim: This study aimed to investigate the correlation between lipin-1 in visceral adipose tissues (VATs) and lipin-2 in the liver. It also examined hepatic T1 values using T1 mapping in IUGR rats.
Objective: The objective of this study was to explore the metabolic mechanisms linking IUGR and adult obesity by analyzing molecular and imaging markers.
Methods: Pregnant rats were fed either a low-protein diet (10%) to induce IUGR or a normal-protein diet (21%) as a control. Male offspring underwent conventional magnetic resonance imaging and native T1 mapping using a 3.0 T whole-body MR scanner at days 21, 56, and 84 post-birth. Liver tissues and VATs were collected for analysis. Lipin-1 and lipin-2 expression levels were measured using Western blot and real-time quantitative PCR.
Results: The IUGR group exhibited significantly higher mRNA and protein expression levels of lipin-1 and lipin-2 compared to the control group at days 21, 56, and 84 after birth. Additionally, the IUGR group demonstrated significantly higher hepatic T1 values than the control group at the corresponding time points. Positive correlations were observed between the protein and mRNA expression levels of lipin-1 and hepatic T1 values. Similarly, the protein and mRNA expression levels of lipin-2 were positively correlated with hepatic T1 values. All results were statistically significant (P<0.05).
Conclusion: The upregulation of lipin-1 and lipin-2 expressions was found to be linked to elevated hepatic T1 values, potentially contributing to adult obesity in IUGR rats.
期刊介绍:
Current Medical Imaging Reviews publishes frontier review articles, original research articles, drug clinical trial studies and guest edited thematic issues on all the latest advances on medical imaging dedicated to clinical research. All relevant areas are covered by the journal, including advances in the diagnosis, instrumentation and therapeutic applications related to all modern medical imaging techniques.
The journal is essential reading for all clinicians and researchers involved in medical imaging and diagnosis.