Joel Johnson George, Sierra H Ball, Elena Wang, Remy T Schneider, Claudia Capdevila, Francesca Martini, Hyeonjeong Lee, Jonathan Miller, Liang Cheng, John W Murray, Hans-Willem Snoeck, Kelley S Yan
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引用次数: 0
Abstract
Histological analysis of intestinal epithelial tissues is enhanced by 3D visualization compared to 2D sections. Here, we present a protocol for 3D visualization of intestinal epithelial cells using an optical clearing approach optimized for endogenous fluorescence and preservation of crypt-villus morphology. We describe steps for clearing and refractive index matching tissue. We provide detailed procedures for imaging and reconstructing tissue to visualize epithelial cells along the crypt-villus axis with high resolution. We illustrate this approach with endogenous tdTomato used for lineage tracing in the small intestine of Fgfbp1-CreERT2; Rosa26-tdTomato mice. For complete details on the use and execution of this protocol, please refer to Capdevila et al.1.
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