ZC3HC1 has functions distinct from TPR and is dispensable for TPR localisation to the nuclear basket.

Q1 Medicine

Wellcome Open Research Pub Date : 2025-04-11 eCollection Date: 2025-01-01 DOI:10.12688/wellcomeopenres.23711.1

Bethany M Bartlett, Juan Carlos Acosta, Wendy A Bickmore

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引用次数: 0

Abstract

Background: The nuclear basket is a 'fishtrap'-like structure on the nucleoplasmic face of the nuclear pore complex which has been implicated in diverse functions including RNA export, heterochromatin organisation, and mitosis. Recently, a novel component of the nuclear basket, ZC3HC1, has been described. The localisation of ZC3HC1 to nuclear pores has been reported to occur reciprocally with TPR, a major structural component of the nuclear basket.

Methods: Using siRNA-mediated knock down, immunofluorescence and RNA sequencing we compare the consequences of depleting two proteins of the nuclear pore basket - TPR and ZC3HC1.

Results: We show that in human fibroblasts, although ZC3HC1 localisation to nuclear pores is TPR-dependent, TPR localises to pores regardless of the presence of ZC3HC1. We demonstrate that knockdown of TPR and ZC3HC1 produce distinct transcriptional profiles.

Conclusions: Our results suggest that there is little overlap in function between these two nuclear basket proteins in human diploid fibroblasts.

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ZC3HC1具有与TPR不同的功能，对于TPR定位到核篮子是必不可少的。

背景：核筐是核孔复合体核质表面的一个“鱼钩”状结构，它与RNA输出、异染色质组织和有丝分裂等多种功能有关。最近，一种新的核篮子成分，ZC3HC1，已经被描述。据报道，ZC3HC1与核孔的定位与核篮子的主要结构成分TPR相互作用。方法：利用sirna介导的敲低、免疫荧光和RNA测序，我们比较了两种核孔筐蛋白TPR和ZC3HC1的消耗后果。结果：我们发现，在人类成纤维细胞中，尽管ZC3HC1定位到核孔依赖于TPR，但无论ZC3HC1是否存在，TPR都定位到孔中。我们证明TPR和ZC3HC1的敲低产生不同的转录谱。结论：我们的研究结果表明，在人类二倍体成纤维细胞中，这两种核筐蛋白在功能上几乎没有重叠。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Wellcome Open Research Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

5.50

自引率

0.00%

发文量

426

审稿时长

1 weeks

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