Unraveling lncRNA TRG-AS1: a novel biomarker for poor prognosis of gastric cancer and key to regulating malignant behaviors by targeting miR-873-5p.

IF 2.5 3区生物学

Hereditas Pub Date : 2025-05-29 DOI:10.1186/s41065-025-00459-8

Miao Hu, Tiesong Zhang, Yuzhen Ma, Huiling Wang, Suping Hou

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引用次数: 0

Abstract

Background and study aims: To alleviate patient stress and advance gastric cancer research, this study aims to investigate the potential association between aberrant expression of TRG-AS1 and gastric cancer, and to examine its potential impact on the biological behaviors of gastric cancer cells.

Materials and methods: To find out how TRG-AS1 is expressed in the tissues and cells of gastric cancer, real-time fluorescence quantitative PCR was employed. The connection between TRG-AS1 and pathological features, as well as its prognostic importance, were examined using the Chi-square test and Cox regression analysis. The dual luciferase reporting assay was utilized to confirm the targeting of TRG-AS1 and miR-873-5p. Transwell assay and CCK-8 test were used to identify the roles that TRG-AS1 plays in cell metastasis and proliferation, respectively.

Results: Research has revealed a downregulation of TRG-AS1 in the tissues and cells, which is strongly correlated with the differentiation, TNM stage, lymph node metastasis, depth of invasion, and patient survival rate in gastric cancer. The binding sites exists between miR-873-5p and TRG-AS1, and TRG-AS1 has the ability to negatively control miR-873-5p by acting as a competitive endogenous RNA. When TRG-AS1 was overexpressed, the malignant behavioral activity of gastric cancer cells was significantly decreased; however, the inhibitory effect was reversed when miR-873-5p was overexpressed.

Conclusions: TRG-AS1 is a potential predictor of poor prognosis in gastric cancer patients and targets miR-873-5p to inhibit the progression of cancer.

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lncRNA TRG-AS1：胃癌不良预后新标志物及靶向miR-873-5p调控恶性行为的关键

背景与研究目的：为缓解患者应激，推进胃癌研究，本研究旨在探讨TRG-AS1异常表达与胃癌的潜在关联，并探讨其对胃癌细胞生物学行为的潜在影响。材料与方法：为了解TRG-AS1在胃癌组织细胞中的表达情况，采用实时荧光定量PCR技术。采用卡方检验和Cox回归分析检验TRG-AS1与病理特征的关系及其对预后的重要性。采用双荧光素酶报告法确认TRG-AS1和miR-873-5p的靶向性。Transwell法和CCK-8法分别检测了TRG-AS1在细胞转移和增殖中的作用。结果：研究发现组织细胞中TRG-AS1表达下调，与胃癌的分化、TNM分期、淋巴结转移、浸润深度及患者生存率密切相关。结合位点存在于miR-873-5p和TRG-AS1之间，TRG-AS1具有作为竞争性内源性RNA负向控制miR-873-5p的能力。TRG-AS1过表达时，胃癌细胞的恶性行为活性显著降低；然而，当miR-873-5p过表达时，抑制作用被逆转。结论：TRG-AS1是胃癌患者预后不良的潜在预测因子，可靶向miR-873-5p抑制癌症进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.