Enterococcus faecalis strains derived from wild bird provide protection against Clostridium perfringens challenge in locally-sourced broilers.

IF 2.6 2区农林科学 Q1 VETERINARY SCIENCES

Frontiers in Veterinary Science Pub Date : 2025-05-15 eCollection Date: 2025-01-01 DOI:10.3389/fvets.2025.1601605

Gerui Zhang, Ainul Zahra, Tianrui Yang, Qiyu Guo, Yan Sun, Yuhang Zhang, Yupeng Gao, Yuxi Zhang, Mingyue Wang, Jingshuo Gong, Haibin Huang, Zhannan Wang, Chunfeng Wang, Yanlong Jiang

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引用次数: 0

Abstract

Introduction: Necrotic enteritis (NE), caused by Clostridium perfringens, has seen a surge in chicken populations recently due to the ban on antibiotic growth promoters in feed.

Methods: In this research, screening and identification of probiotics with strong antagonistic ability against C. perfringens from 34 wild bird fecal isolates, followed by analysis of probiotic characteristics and carbon source metabolic activity. Strains exhibiting favorable antagonistic activity against C. perfringens were subsequently employed in vivo study to evaluate their protective efficacy against C. perfringens challenge in locally-sourced broilers.

Results: The results showed that Enterococcus faecalis strains YL-EF25 and YL-EF32 were selected based on their ability to inhibit the growth and biofilm formation of C. perfringens. These two strains demonstrated good tolerance to bile salts, artificial gastric juice, and phenol, as well as metabolic activity toward dietary fiber and propionic acid precursor substances. In vivo tests on locally-sourced broilers revealed that NE induced body weight loss, intestinal lesions, and intestinal inflammation, as well as imbalance in the gut microflora. Administration of E. faecalis YL-EF25 and YL-EF32 can alleviate these symptoms. We find that feed supplementation with YL-EF25 and YL-EF32 reduced the lesion score of challenged chicks (p < 0.05), with increased tight junction-related gene expression (Occludin and ZO-1) and decreased proinflammatory cytokine (TNF-α and IFN-γ) expression in jejunum compared with NE-induced broilers (p < 0.05). Furthermore, E. faecalis YL-EF25 can boost peripheral blood lymphocyte proliferation activity (p < 0.05).

Discussion: These finding indicated that addition of E. faecalis YL-EF25 and YL-EF32 improved growth performance and mitigated NE-induced gut injury, possibly by strengthening intestinal mucosal barrier function and restoring effects on the ileal microbial composition in C. perfringens-challenged broilers.

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来自野鸟的粪肠球菌菌株对本地肉鸡产气荚膜梭状芽胞杆菌的攻击具有保护作用。

导语：由于禁止在饲料中添加抗生素生长促进剂，由产气荚膜梭菌引起的坏死性肠炎（NE）最近在鸡群中激增。方法：本研究从34株野鸟粪便中筛选出对产气荚膜荚膜杆菌具有较强拮抗能力的益生菌，并进行益生菌特性和碳源代谢活性分析。对产气荚膜荚膜杆菌表现出良好拮抗活性的菌株进行了体内研究，以评估其对本地肉鸡产气荚膜荚膜杆菌攻击的保护作用。结果：以粪肠球菌YL-EF25和YL-EF32抑制产气荚膜荚膜球菌生长和生物膜形成的能力为基础，筛选了粪肠球菌菌株YL-EF25和YL-EF32。这两个菌株对胆盐、人工胃液和苯酚具有良好的耐受性，对膳食纤维和丙酸前体物质具有良好的代谢活性。对本地肉鸡的体内试验表明，NE会导致体重减轻、肠道病变和肠道炎症，以及肠道菌群失衡。使用粪肠杆菌YL-EF25和YL-EF32可减轻这些症状。结果表明，与ne诱导的肉鸡相比，添加YL-EF25和YL-EF32降低了攻毒雏鸡的病变评分（p < 0.05），提高了空肠紧密连接相关基因Occludin和ZO-1的表达，降低了促炎细胞因子TNF-α和IFN-γ的表达（p < 0.05）。粪肠杆菌YL-EF25对小鼠外周血淋巴细胞增殖活性有促进作用（p < 0.05）。讨论：这些发现表明，添加粪肠杆菌YL-EF25和YL-EF32可以改善产气荚膜梭菌肉鸡的生长性能，减轻ne诱导的肠道损伤，可能是通过增强肠黏膜屏障功能和恢复对回肠微生物组成的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Veterinary Science Veterinary-General Veterinary

CiteScore

4.80

自引率

9.40%

发文量

1870

审稿时长

14 weeks

期刊介绍： Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.