{"title":"A Pentatricopeptide Repeat Protein Restores Fertility in Tadukan-Type Cytoplasmic Male Sterile Rice via the Cleavage of the Mitochondrial orf312 RNA.","authors":"Ayumu Takatsuka, Yuko Iwai, Hakim Mireau, Tomohiko Kazama, Hiroyuki Ichida, Tomoko Abe, Keisuke Igarashi, Kinya Toriyama","doi":"10.1111/ppl.70308","DOIUrl":null,"url":null,"abstract":"<p><p>Cytoplasmic male sterility (CMS) is associated with the inhibition of pollen and/or anther development regulated by a CMS-causing gene in the mitochondrial genome; it is a useful trait for preventing self-pollination and producing F<sub>1</sub> hybrids, which can boost crop yields. Pollen and/or anther development can be recovered by the action of the RESTORER OF FERTILITY (Rf) gene, a nuclear-encoded gene. Most reported Rf genes encode pentatricopeptide repeat (PPR) proteins, which bind to RNA and promote RNA processing of the respective CMS-causing gene. In this study, we report the map-based cloning of the Rf gene (Rfta) for Tadukan-type CMS (TA-CMS) in rice (Oryza sativa L.), with anther dehiscence and seed setting inhibited by the mitochondrial gene orf312. The Rfta locus was delimited to a region comprising 10 PPR genes forming a cluster on chromosome 10. The complementation test revealed that the introduction of a PPR gene, PPR796, into the TA-CMS line resulted in the recovery of the anther dehiscence and seed setting. RNA-gel blot analysis and the determination of 3' ends of the orf312 RNA confirmed the PPR796-mediated cleavage of the orf312 RNA in the transgenic TA-CMS line. Furthermore, RNA gel electrophoretic mobility shift assays revealed that the recombinant PPR796 protein bound to the 3' side of the orf312 RNA in vitro. We concluded that RFta/PPR796 binds to orf312 RNA and promotes RNA cleavage to restore fertility in TA-CMS.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 3","pages":"e70308"},"PeriodicalIF":5.4000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123059/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70308","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Cytoplasmic male sterility (CMS) is associated with the inhibition of pollen and/or anther development regulated by a CMS-causing gene in the mitochondrial genome; it is a useful trait for preventing self-pollination and producing F1 hybrids, which can boost crop yields. Pollen and/or anther development can be recovered by the action of the RESTORER OF FERTILITY (Rf) gene, a nuclear-encoded gene. Most reported Rf genes encode pentatricopeptide repeat (PPR) proteins, which bind to RNA and promote RNA processing of the respective CMS-causing gene. In this study, we report the map-based cloning of the Rf gene (Rfta) for Tadukan-type CMS (TA-CMS) in rice (Oryza sativa L.), with anther dehiscence and seed setting inhibited by the mitochondrial gene orf312. The Rfta locus was delimited to a region comprising 10 PPR genes forming a cluster on chromosome 10. The complementation test revealed that the introduction of a PPR gene, PPR796, into the TA-CMS line resulted in the recovery of the anther dehiscence and seed setting. RNA-gel blot analysis and the determination of 3' ends of the orf312 RNA confirmed the PPR796-mediated cleavage of the orf312 RNA in the transgenic TA-CMS line. Furthermore, RNA gel electrophoretic mobility shift assays revealed that the recombinant PPR796 protein bound to the 3' side of the orf312 RNA in vitro. We concluded that RFta/PPR796 binds to orf312 RNA and promotes RNA cleavage to restore fertility in TA-CMS.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.