A Pentatricopeptide Repeat Protein Restores Fertility in Tadukan-Type Cytoplasmic Male Sterile Rice via the Cleavage of the Mitochondrial orf312 RNA.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Ayumu Takatsuka, Yuko Iwai, Hakim Mireau, Tomohiko Kazama, Hiroyuki Ichida, Tomoko Abe, Keisuke Igarashi, Kinya Toriyama
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引用次数: 0

Abstract

Cytoplasmic male sterility (CMS) is associated with the inhibition of pollen and/or anther development regulated by a CMS-causing gene in the mitochondrial genome; it is a useful trait for preventing self-pollination and producing F1 hybrids, which can boost crop yields. Pollen and/or anther development can be recovered by the action of the RESTORER OF FERTILITY (Rf) gene, a nuclear-encoded gene. Most reported Rf genes encode pentatricopeptide repeat (PPR) proteins, which bind to RNA and promote RNA processing of the respective CMS-causing gene. In this study, we report the map-based cloning of the Rf gene (Rfta) for Tadukan-type CMS (TA-CMS) in rice (Oryza sativa L.), with anther dehiscence and seed setting inhibited by the mitochondrial gene orf312. The Rfta locus was delimited to a region comprising 10 PPR genes forming a cluster on chromosome 10. The complementation test revealed that the introduction of a PPR gene, PPR796, into the TA-CMS line resulted in the recovery of the anther dehiscence and seed setting. RNA-gel blot analysis and the determination of 3' ends of the orf312 RNA confirmed the PPR796-mediated cleavage of the orf312 RNA in the transgenic TA-CMS line. Furthermore, RNA gel electrophoretic mobility shift assays revealed that the recombinant PPR796 protein bound to the 3' side of the orf312 RNA in vitro. We concluded that RFta/PPR796 binds to orf312 RNA and promotes RNA cleavage to restore fertility in TA-CMS.

一种五肽重复蛋白通过切割线粒体orf312 RNA恢复tadukan型细胞质雄性不育水稻的育性。
细胞质雄性不育(CMS)与线粒体基因组中引起CMS的基因调控的花粉和/或花药发育抑制有关;它是防止自花授粉和生产F1杂交品种的有用性状,可以提高作物产量。花粉和/或花药的发育可以通过一个核编码基因——育性恢复基因(Rf)的作用来恢复。大多数报道的Rf基因编码五肽重复(PPR)蛋白,该蛋白与RNA结合并促进相应cms引起基因的RNA加工。本研究报道了线粒体基因orf312抑制水稻tadukan型CMS (TA-CMS)花药开裂和结实率的Rf基因(Rfta)图谱克隆。Rfta位点在10号染色体上由10个PPR基因组成一个簇。互补试验表明,将PPR基因PPR796导入TA-CMS系后,花药开裂和结实率恢复。RNA-凝胶印迹分析和对orf312 RNA 3'端的测定证实了ppr796介导的orf312 RNA在转基因TA-CMS系中的裂解。此外,RNA凝胶电泳迁移转移实验表明,重组PPR796蛋白在体外结合到orf312 RNA的3'侧。我们得出结论,RFta/PPR796结合orf312 RNA并促进RNA裂解以恢复TA-CMS的生育能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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