Analysis of Specific MicroRNAs Expressed after Intake of Cobalt and Xenon Preparations for Anti-Doping Control.

IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
P V Postnikov, I V Pronina, D S Polyansky, Yu A Efimova, Z G Ordzhonikidze, V A Badtieva, D B Nikityuk
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引用次数: 0

Abstract

Xenon and cobalt are representatives of hypoxia-inducible factor (HIF) activators stimulating erythropoiesis and are prohibited by the World Anti-Doping Agency (WADA). The expression profiles of 84 microRNAs circulating in blood plasma and involved in the regulation of the HIF-1α transcription factor and its downstream signaling pathways were analyzed in volunteers before and after intake of cobalt (II) and xenon preparations using quantitative real-time PCR and certified miRCURY LNA miRNA Focus Panel (Qiagen) for analysis of microRNA expression profiles. Intake of cobalt aspartate increased the expression of hsa-miR-15b-5p, while inhalation of the Xe/O2 mixture significantly increased the plasma levels of hsa-miR-378a-3p and hsa-miR-491-5p. Thus, we for the first time revealed differential expression of microRNAs circulating in plasma in response to various HIF activators. Potential microRNA markers for identification of cobalt and xenon abuse for anti-doping control are proposed.

用于反兴奋剂控制的钴和氙制剂摄入后表达的特异性microrna分析。
氙和钴是刺激红细胞生成的缺氧诱导因子(HIF)激活剂的代表,被世界反兴奋剂机构(WADA)禁止使用。采用实时荧光定量PCR和认证miRCURY LNA miRNA Focus Panel (Qiagen)分析志愿者在摄入钴(II)和氙制剂前后血浆中循环的84种参与HIF-1α转录因子及其下游信号通路调控的microRNA表达谱。摄入天冬氨酸钴增加了hsa-miR-15b-5p的表达,而吸入Xe/O2混合物显著增加了hsa-miR-378a-3p和hsa-miR-491-5p的血浆水平。因此,我们首次揭示了血浆中循环的microrna对各种HIF激活剂的差异表达。提出了用于反兴奋剂控制的钴和氙滥用的潜在microRNA标记物。
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来源期刊
Bulletin of Experimental Biology and Medicine
Bulletin of Experimental Biology and Medicine 医学-医学:研究与实验
CiteScore
1.50
自引率
14.30%
发文量
265
审稿时长
2 months
期刊介绍: Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.
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