Unraveling Tissue-Specific Fatty Acid Biosynthesis and Inter-Tissue Crosstalk in Mice through Stable-Isotope Tracing Metabolomics.

IF 14.3 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Advanced Science Pub Date : 2025-05-30 DOI:10.1002/advs.202503662

Beizi Xing, Ruohong Wang, Tianzhang Kou, Wenbin Liu, Zheng-Jiang Zhu

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引用次数: 0

Abstract

Biosynthesis of free fatty acids (FFAs) in mammals is pivotal for metabolic homeostasis, yet a comprehensive understanding of tissue-specific biosynthesis and inter-tissue crosstalk of FFAs remains incomplete. In vivo stable-isotope tracing metabolomics is utilized to comprehensively measure FFA biosynthesis and inter-tissue crosstalk in mice. Systematically assessing tissue-specific biosynthesis of 13 FFAs across 15 tissues unveils dynamic spatial and temporal accumulation and redistribution of FFAs throughout the body. Employing an analytical framework to deconvolve mass isotopologue patterns, inter-tissue crosstalk is explored for saturated, polyunsaturated, and monounsaturated FFAs, and quantify communications of FFA (16:0) and FFA (18:0) between the liver and other tissues. Then, a decline in fatty acid biosynthesis in peripheral tissues but not in the brain of aged mice is observed, particularly evident in palmitic acid and monounsaturated fatty acids. These findings illuminate the complex interplay between tissue-specific fatty acid biosynthesis and the maintenance of metabolic homeostasis.

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通过稳定同位素示踪代谢组学揭示小鼠组织特异性脂肪酸生物合成和组织间串扰。

哺乳动物游离脂肪酸（FFAs）的生物合成对代谢稳态至关重要，但对组织特异性游离脂肪酸的生物合成和组织间串扰的全面了解仍不完整。体内稳定同位素示踪代谢组学用于综合测量小鼠FFA生物合成和组织间串扰。系统地评估15个组织中13种游离脂肪酸的组织特异性生物合成，揭示了游离脂肪酸在全身的动态时空积累和再分布。采用反褶曲质量同位素模式的分析框架，研究了饱和、多不饱和和单不饱和脂肪酸的组织间串扰，并量化了肝脏与其他组织之间FFA（16:0）和FFA（18:0）的通信。然后，观察到外周组织的脂肪酸生物合成下降，但在老年小鼠的大脑中没有，特别是棕榈酸和单不饱和脂肪酸。这些发现阐明了组织特异性脂肪酸生物合成与代谢稳态维持之间复杂的相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advanced Science CHEMISTRY, MULTIDISCIPLINARYNANOSCIENCE &-NANOSCIENCE & NANOTECHNOLOGY

CiteScore

18.90

自引率

2.60%

发文量

1602

审稿时长

1.9 months

期刊介绍： Advanced Science is a prestigious open access journal that focuses on interdisciplinary research in materials science, physics, chemistry, medical and life sciences, and engineering. The journal aims to promote cutting-edge research by employing a rigorous and impartial review process. It is committed to presenting research articles with the highest quality production standards, ensuring maximum accessibility of top scientific findings. With its vibrant and innovative publication platform, Advanced Science seeks to revolutionize the dissemination and organization of scientific knowledge.