A preliminary study on helminths detected in red foxes (Vulpes vulpes) in Bingol Province of Türkiye: morphological and molecular approaches.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2025-05-30 DOI:10.1186/s12917-025-04838-1

Seyma Gunyakti Kilinc, Harun Kaya Kesik, Figen Celik, Sami Simsek

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引用次数: 0

Abstract

Background: Red foxes (Vulpes vulpes) are hosts to a large number of endoparasites, some of which are zoonotic. This study was carried out to identify and molecularly characterise intestinal helminths in foxes in Bingol province, eastern Türkiye. For this purpose, carcasses of 15 red foxes that died as a result of traffic accidents in Bingol province were obtained. Parasite material obtained from the intestines was identified according to its morphological characteristics, and complete gDNA was isolated from each individual parasite samples. A 446 bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) gene was amplified using PCR. Subsequently, a unidirectional sequence analysis was conducted.

Results: The mt-CO1 gene region of a total of 8 helminth species was successfully sequenced and identified by BLAST searches. One trematode (Alaria alata), five cestodes (Dipylidium caninum, Joyeuxiella sp., Taenia hydatigena, Mesocestoides litteratus, Mesocestoides sp.), one nematode (Toxascaris leonina) and one acanthocephalan (Macracanthorhynchus hirudinaceus) species were identified. Phylogenetic and haplotype analyses were performed on the obtained Mesocestoides spp. sequences. Haplotype analysis of Mesocestoides litteratus isolates revealed 22 haplotypes Mesocestoides.

Conclusion: These findings are important to draw attention to the wild circulation of some zoonotic helminths.

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新疆Bingol省红狐（Vulpes Vulpes）中蠕虫的初步研究：形态学和分子方法。

背景：红狐（Vulpes Vulpes）是大量内寄生虫的宿主，其中一些是人畜共患的。本研究是为了鉴定和分子特征的狐狸肠道蠕虫在Bingol省，东部 rkiye。为此，获得了在Bingol省因交通事故死亡的15只红狐的尸体。从肠道中获得的寄生虫材料根据其形态特征进行鉴定，并从每个寄生虫样本中分离出完整的gDNA。采用PCR扩增出线粒体细胞色素c氧化酶亚基1 （mt-CO1）基因446 bp片段。随后进行了单向序列分析。结果：通过BLAST搜索，成功测序并鉴定了8种蠕虫的mt-CO1基因区。鉴定出吸虫（Alaria alata） 1种，绦虫（Dipylidium caninum, Joyeuxiella sp.），带绦虫（Taenia hyatigena, Mesocestoides literatus, Mesocestoides sp.） 5种，线虫（Toxascaris leonina） 1种，棘头虫（Macracanthorhynchus hirudinaceus） 1种。对所获得的介骨类昆虫序列进行了系统发育和单倍型分析。单倍型分析显示22个单倍型。结论：这些发现对引起人们对某些人畜共患蠕虫野生传播的重视具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.