Bifunctional transcription factor GhMYB4 orchestrates transition from elongation to secondary cell wall synthesis trade-off in cotton fiber

IF 10.7 1区生物学 Q1 CELL BIOLOGY

Developmental cell Pub Date : 2025-05-30 DOI:10.1016/j.devcel.2025.05.004

Lu Qiao, Haozhe Tan, Mengling Sun, Xiao Zhang, Shenghua Xiao, Zhengxiu Ye, Zhiwei Chen, Xianlong Zhang, Lili Tu

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Abstract

The integration of cell wall expansion and reinforcement is vital for plant cell wall development. Cotton fiber is characterized by the synchronized development of fiber growth and cell wall formation, providing an excellent system for investigating plant cell walls. Here, we found that the often-overlooked transition stage coordinates fiber elongation and secondary cell wall (SCW) synthesis through antagonistic effects of the transcription factor GhMYB4. Knockout of GhMYB4 produced longer and finer fibers, contrasting with overexpression of GhMYB4. We show that GhMYB4 represses fiber elongation through fatty acid and brassinosteroid pathways involving a negative feedback loop with GhRAP2 and GhTCP15, while activating SCW synthesis by inducing cellulose biosynthesis in succession with the GhMYB52-GhFSN1-GhILR3 module. We identify that the bifunctionality of GhMYB4 depends on its interaction with different cis-elements and is executed through distinct transcriptional regulation motifs. Our findings propose a strategy to improve fiber quality by orchestrating wall expansion and stiffness.

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双功能转录因子GhMYB4协调棉纤维从伸长到次级细胞壁合成的过渡

细胞壁的扩展和加固的整合对植物细胞壁的发育至关重要。棉纤维具有纤维生长和细胞壁形成同步发展的特点，为研究植物细胞壁提供了良好的系统。在这里，我们发现经常被忽视的过渡阶段通过转录因子GhMYB4的拮抗作用协调纤维伸长和次级细胞壁（SCW）合成。与过表达GhMYB4相比，敲除GhMYB4可产生更长更细的纤维。研究表明，GhMYB4通过脂肪酸和油菜素类固醇途径抑制纤维伸长，其中包括GhRAP2和GhTCP15的负反馈回路，同时通过GhMYB52-GhFSN1-GhILR3模块相继诱导纤维素生物合成来激活SCW合成。我们发现GhMYB4的双重功能取决于它与不同顺式元件的相互作用，并通过不同的转录调控基序来执行。我们的研究结果提出了一种通过协调壁膨胀和刚度来改善纤维质量的策略。

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来源期刊

Developmental cell 生物-发育生物学

CiteScore

18.90

自引率

1.70%

发文量

203

审稿时长

3-6 weeks

期刊介绍： Developmental Cell, established in 2001, is a comprehensive journal that explores a wide range of topics in cell and developmental biology. Our publication encompasses work across various disciplines within biology, with a particular emphasis on investigating the intersections between cell biology, developmental biology, and other related fields. Our primary objective is to present research conducted through a cell biological perspective, addressing the essential mechanisms governing cell function, cellular interactions, and responses to the environment. Moreover, we focus on understanding the collective behavior of cells, culminating in the formation of tissues, organs, and whole organisms, while also investigating the consequences of any malfunctions in these intricate processes.