The PlMYB73-PlMYB70-PlMYB108 complex regulates PlTPS1 to promote geraniol biosynthesis in Paeonia lactiflora

IF 8.7 1区 农林科学 Q1 Agricultural and Biological Sciences
Qian Zhao, Yuqing Li, Lina Gu, Yehua Yang, Di He, Jianrang Luo, Yanlong Zhang
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Abstract

Geraniol contributes significantly to the floral scent of herbaceous peony (Paeonia lactiflora) and is abundant in fragrant cultivars. However, the regulatory mechanism of geraniol biosynthesis in herbaceous peony remains unclear. In this study, we identified a transcriptional regulatory complex (PlMYB73-PlMYB70-PlMYB108) that cooperatively regulated geraniol biosynthesis in herbaceous peony. The three MYB members were identified through correlation analysis between geraniol content and gene expression profiles in 17 herbaceous peony cultivars. Transient overexpression and gene silencing experiments revealed that PlMYB73, PlMYB108, and PlMYB70 positively regulated PlTPS1 expression and geraniol accumulation. PlMYB108 and PlMYB70 directly upregulate PlTPS1 by binding to the TAACCA and CAACTG motifs, respectively, as demonstrated by yeast one-hybrid, dual-luciferase, and electrophoretic mobility shift assays. Although PlMYB73 did not directly bind to the PlTPS1 promoter, yeast two-hybrid, bimolecular fluorescence complementation, luciferase complementation imaging, and dual-luciferase assays revealed its interaction with PlMYB70 in the nucleus, resulting in synergistic activation of PlTPS1. PlMYB108 was also found to interact with PlMYB70. The three MYB transcription factors formed the PlMYB73-PlMYB70-PlMYB108 complex. Gene co-overexpression and co-silencing experiments demonstrated that the complex significantly enhanced geraniol biosynthesis. In conclusion, our research provides novel insights into the molecular mechanism by which transcription factors cooperatively regulate geraniol biosynthesis.
PlMYB73-PlMYB70-PlMYB108复合物调节PlTPS1促进芍药中香叶醇的生物合成
香叶醇对草本牡丹(芍药)的花香有重要贡献,在芳香品种中含量丰富。然而,芍药中香叶醇生物合成的调控机制尚不清楚。在这项研究中,我们鉴定了一个转录调控复合物(PlMYB73-PlMYB70-PlMYB108),该复合物协同调节草本牡丹中香叶醇的生物合成。通过对17个芍药品种香叶醇含量与基因表达谱的相关性分析,鉴定出3个MYB成员。短暂过表达和基因沉默实验表明,PlMYB73、PlMYB108和PlMYB70正调控PlTPS1的表达和香叶醇的积累。PlMYB108和PlMYB70分别通过结合TAACCA和CAACTG基序直接上调PlTPS1,酵母单杂交、双荧光素酶和电泳迁移转移实验证明了这一点。虽然PlMYB73不直接结合PlTPS1启动子,但酵母双杂交、双分子荧光互补、荧光素酶互补成像和双荧光素酶检测显示其在细胞核内与PlMYB70相互作用,导致PlTPS1协同活化。PlMYB108也被发现与PlMYB70相互作用。这三个MYB转录因子组成了PlMYB73-PlMYB70-PlMYB108复合体。基因共过表达和共沉默实验表明,该复合物显著促进了香叶醇的生物合成。总之,我们的研究为转录因子协同调节香叶醇生物合成的分子机制提供了新的见解。
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来源期刊
Horticulture Research
Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
11.20
自引率
6.90%
发文量
367
审稿时长
20 weeks
期刊介绍: Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.
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