Association of Insertion/Deletion Polymorphisms of MDM2, MCP-1 and VEGF with Esophageal Cancer Risk in North-West Indians: A Case - Control Study.

Q2 Medicine

Asian Pacific Journal of Cancer Prevention Pub Date : 2025-05-01 DOI:10.31557/APJCP.2025.26.5.1623

Deepanshi Mahajan, Vasudha Sambyal, Manjit Singh Uppal, Meena Sudan, Kamlesh Guleria

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引用次数: 0

Abstract

Background: The carcinogenesis process in esophageal cancer (EC), a highly heterogeneous and multifaceted disease, is influenced by both tumor angiogenesis and chronic inflammation pathways. Genetic variants in these pathways may affect the progression and development of EC, ultimately contributing to different susceptibilities to cancer among individuals.

Methods: In this study, a total of 536 subjects were recruited, including 260 EC patients and 276 healthy individuals. The DNA was isolated from the blood samples of the participants using the standard phenol-chloroform method. The three insertion/deletion (ins/del) polymorphisms (VEGF-2549 18bp I/D, MDM2 40bp I/D, and MCP-1 14bp I/D) were screened using the Direct-polymerase chain reaction (PCR) genotyping method. The role of gene-environment interactions on EC risk was assessed using the Multifactor Dimensionality Reduction (MDR) software (version 3.0.2).

Results: It was observed that the individuals carrying the II genotype and I allele of the VEGF-2549 18bp I/D polymorphism, as well as the carriers of the ID and DD genotypes and D allele of the MCP-1 14bp I/D polymorphism had a higher risk of developing EC. No association between the MDM2 40bp I/D polymorphism and EC risk was reported in this study. Genotype combination analysis revealed an increased EC risk in the carriers of the II-II-II genotype combination of the VEGF-2549 18bp I/D, MDM2 40bp I/D, and MCP-1 14bp I/D polymorphisms compared to those with other genotype combinations. The gene-environment interaction analysis also indicated a strong interaction between lifestyle factors and genetic polymorphisms in influencing EC risk.

Conclusion: The present study concluded that the VEGF-2549 18bp I/D and MCP-1 14bp I/D variants were associated with EC risk in the North-west Indians.

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西北印度人MDM2、MCP-1和VEGF插入/缺失多态性与食管癌风险的关联：一项病例对照研究

背景：食管癌（EC）是一种高度异质性和多面性的疾病，其癌变过程同时受到肿瘤血管生成和慢性炎症途径的影响。这些途径中的遗传变异可能影响EC的进展和发展，最终导致个体对癌症的不同易感性。方法：本研究共招募536名受试者，其中EC患者260例，健康个体276例。DNA是用标准的苯酚-氯仿法从参与者的血液样本中分离出来的。采用直接聚合酶链反应（PCR）基因分型方法筛选3个插入/缺失（ins/del）多态性（VEGF-2549 18bp I/D、MDM2 40bp I/D和MCP-1 14bp I/D）。使用多因素降维（MDR）软件（版本3.0.2）评估基因-环境相互作用对EC风险的作用。结果：携带VEGF-2549 18bp I/D多态性II基因型和I等位基因的个体，以及携带MCP-1 14bp I/D多态性ID和DD基因型和D等位基因的个体发生EC的风险较高。本研究未发现MDM2 40bp I/D多态性与EC风险相关。基因型组合分析显示，与其他基因型组合相比，VEGF-2549 18bp I/D、MDM2 40bp I/D和MCP-1 14bp I/D多态性的II-II-II基因型组合携带者的EC风险增加。基因-环境相互作用分析还表明，生活方式因素和遗传多态性在影响EC风险方面存在很强的相互作用。结论：本研究得出结论，VEGF-2549 18bp I/D和MCP-1 14bp I/D变异与西北印度人的EC风险相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Pacific Journal of Cancer Prevention 医学-肿瘤学

CiteScore

2.80

自引率

0.00%

发文量

779

审稿时长

3 months

期刊介绍： Cancer is a very complex disease. While many aspects of carcinoge-nesis and oncogenesis are known, cancer control and prevention at the community level is however still in its infancy. Much more work needs to be done and many more steps need to be taken before effective strategies are developed. The multidisciplinary approaches and efforts to understand and control cancer in an effective and efficient manner, require highly trained scientists in all branches of the cancer sciences, from cellular and molecular aspects to patient care and palliation. The Asia Pacific Organization for Cancer Prevention (APOCP) and its official publication, the Asia Pacific Journal of Cancer Prevention (APJCP), have served the community of cancer scientists very well and intends to continue to serve in this capacity to the best of its abilities. One of the objectives of the APOCP is to provide all relevant and current scientific information on the whole spectrum of cancer sciences. They aim to do this by providing a forum for communication and propagation of original and innovative research findings that have relevance to understanding the etiology, progression, treatment, and survival of patients, through their journal. The APJCP with its distinguished, diverse, and Asia-wide team of editors, reviewers, and readers, ensure the highest standards of research communication within the cancer sciences community across Asia as well as globally. The APJCP publishes original research results under the following categories: -Epidemiology, detection and screening. -Cellular research and bio-markers. -Identification of bio-targets and agents with novel mechanisms of action. -Optimal clinical use of existing anti-cancer agents, including combination therapies. -Radiation and surgery. -Palliative care. -Patient adherence, quality of life, satisfaction. -Health economic evaluations.