Mismatch Repair Proteins Immunostaining in Lip Squamous Cell Carcinoma: A Role in Lip Carcinogenesis?

Q2 Medicine

Asian Pacific Journal of Cancer Prevention Pub Date : 2025-05-01 DOI:10.31557/APJCP.2025.26.5.1553

Yamyle Velasquez Barragán, Anna Clara Aragao Matos Carlos, Gabriella Alves Juliao Costa, Osias Vieira Oliveira Filho, Thâmara Manoela Marinho Bezerra, Sergio Ferreira Juaçaba, Thinali Sousa Dantas, Ana Paula Negreiros Nunes Alves, Paulo Goberlânio De Barros Silva

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引用次数: 0

Abstract

Objective: Lip squamous cell carcinoma (LSCC) is associated with malignant transformation of actinic cheilitis (AC). Since solar radiation alters the functions of mismatch repair (MMR) complex, we evaluated for their possible role in lip carcinogenesis.

Materials and methods: Samples of normal lip epithelia (NLE) (n=15), AC (n=30), and LSCC (n=45) were subjected to immunohistochemistry for MutSα (MSH2/MSH6) and MutLα (MLH1/PMS2) to assess the percentage (brown nuclei over all the keratinocytes in NLE and AC or all tumoral cells in LSCC) of nuclear positive cells and MSH2/MSH6 (MutSα-imbalance) and MLH1/PMS2 (MutLα-imbalance) ratios. Clinical-prognostic variables of the primary tumor and histopathological gradation (LSCC and AC) were evaluated. Mann-Whitney, Kruskal-Wallis/Dunn, and Spearman correlation tests were used (p<0.05, SPSS 20.0).

Results: LSCC and AC showed significant increases in MSH2 (p<0.001), MSH6 (p<0.001), MLH1 (p=0.040) percentage of immunostained cells, and MutSα-imbalance (p<0.001). MutSα-imbalance in AC was higher than MutLα-imbalance (p=0.028). In LSCC, T3/T4 tumors showed higher MutSα-imbalance (p=0.028) and MutLα-imbalance (p=0.014). In LSCC with nodal metastasis, the MutLα-imbalance was significantly higher than the MutSα-imbalance (p=0.046). AC with high-risk dysplasia (p=0.024) and LSCC with vascular invasion (p=0.035) showed lower immunostaining for MSH6. Direct correlations between MMR-proteins increased in LSCC.

Conclusions: Increased MMR expression in lip cancer and the imbalance between MutSa and MutLα is associated with the progression and prognosis of LSCC.

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错配修复蛋白免疫染色在唇部鳞状细胞癌中的作用？

目的：唇鳞状细胞癌（LSCC）与光化性唇炎（AC）的恶性转化有关。由于太阳辐射改变了错配修复（MMR）复合体的功能，我们评估了它们在唇部癌变中的可能作用。材料和方法：对正常唇上皮（NLE）（n=15）、AC （n=30）和LSCC （n=45）进行MutSα (MSH2/MSH6)和MutLα (MLH1/PMS2)的免疫组化，评估核阳性细胞的百分比（NLE和AC中所有角质形成细胞的棕色细胞核或LSCC中所有肿瘤细胞）和MSH2/MSH6 （MutSα-失衡）和MLH1/PMS2 （MutLα-失衡）的比例。评估原发肿瘤的临床预后变量和组织病理学分级（LSCC和AC）。采用Mann-Whitney、Kruskal-Wallis/Dunn和Spearman相关检验（结果：LSCC和AC均显示MSH2显著升高）。结论：MMR在唇癌中的表达升高以及MutSa和MutLα之间的失衡与LSCC的进展和预后有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Pacific Journal of Cancer Prevention 医学-肿瘤学

CiteScore

2.80

自引率

0.00%

发文量

779

审稿时长

3 months

期刊介绍： Cancer is a very complex disease. While many aspects of carcinoge-nesis and oncogenesis are known, cancer control and prevention at the community level is however still in its infancy. Much more work needs to be done and many more steps need to be taken before effective strategies are developed. The multidisciplinary approaches and efforts to understand and control cancer in an effective and efficient manner, require highly trained scientists in all branches of the cancer sciences, from cellular and molecular aspects to patient care and palliation. The Asia Pacific Organization for Cancer Prevention (APOCP) and its official publication, the Asia Pacific Journal of Cancer Prevention (APJCP), have served the community of cancer scientists very well and intends to continue to serve in this capacity to the best of its abilities. One of the objectives of the APOCP is to provide all relevant and current scientific information on the whole spectrum of cancer sciences. They aim to do this by providing a forum for communication and propagation of original and innovative research findings that have relevance to understanding the etiology, progression, treatment, and survival of patients, through their journal. The APJCP with its distinguished, diverse, and Asia-wide team of editors, reviewers, and readers, ensure the highest standards of research communication within the cancer sciences community across Asia as well as globally. The APJCP publishes original research results under the following categories: -Epidemiology, detection and screening. -Cellular research and bio-markers. -Identification of bio-targets and agents with novel mechanisms of action. -Optimal clinical use of existing anti-cancer agents, including combination therapies. -Radiation and surgery. -Palliative care. -Patient adherence, quality of life, satisfaction. -Health economic evaluations.